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RGS·His Antibody

For sensitive detection of His-tagged proteins with an RGS·His epitope

  • Highly specific detection of C-terminal, N-terminal, and internal His tags
  • Highly sensitive and specific detection of RGS·His epitopes

Monoclonal mouse Anti·His Antibodies, like the Anti·His Antibody, are used to detect recombinant protein carrying His tags. RGS·His is one of three variants, each of which detects a different His tag epitope. Like all QIAGEN mouse monoclonal antibodies, RGS·His is prepared under serum-free conditions, which guarantee the absence of viruses, mycoplasma, and contaminating immunoglobulins.

Cat No./ID: 34610
RGS·His Antibody (100 μg)
$697.00
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100 μg mouse anti·RGS(H)4 (lyophilized, with BSA, for 1000 ml working solution)
The RGS·His Antibody is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Performance
Performance of RGS·His Antibody
PropertyValue
Detection: N-terminal, C-terminal, and internal His tags (containing the epitope RGSHHHH)
Sensitivity in dot blots:* 0.5 ng
Sensitivity in western blots:* 2 ng
Isotype: Mouse IgG1
Cross-reactivity: Negligible with crude E. coli, yeast, mammalian, or insect cell lysates
Form: Lyophilized
Working solution: 1000 ml
* Detection using chromogenic substrate. Sensitivity may vary with different proteins.

Many different proteins have been detected, via their His tags, using the QIAexpress Anti·His Antibodies (see table). For the sensitivity of the three Anti·His Antibodies in detecting this panel of proteins see the figure Sensitivity of QIAexpress Anti·His Antibodies)

Proteins tested with QIAexpress Anti·His Antibodies

Protein  Tag locationSequence
A DHFR N-terminus MRGSHHHHHHGSVRPLNCIV...
B DHFR C-terminus ...VYEKKDGSRSHHHHHH
C Thioredoxin N-terminus MRGSHHHHHHGSSDKIIHLT...
D TNFα N-terminus MRHHHHHHQSTESMIRDV...
E TFIIAγ N-terminus MGSSHHHHHHSSGLVPRGSH...
F Chaperonin C-terminus ...AKIAKDGSRSHHHHHH
G DNA Polymerase C-terminus ...EDWLSAKERSHHHHHH
H TFIIAαβ Internal ...QQQHQPQQQQHHHHHHQQAQPQQTVP...
Principle
All QIAexpress mouse monoclonal antibodies are produced under conditions that guarantee the highest purity and activity. Hybridoma cell culture is carried out under serum-free conditions, ensuring that the preparations are free of viruses, mycoplasma, and contaminating immunoglobulins. Purification by adsorption chromatography is performed entirely at physiological pH, yielding preparations with the highest proportion of native antibody.
Procedure
The QIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.
Applications

QIAexpress Detection Systems, with Anti·His Antibodies, allow efficient detection of His-tagged proteins and are ideal for many applications, including:

Colony, dot, and western blotting procedures 
Screening for positive expression clones
Monitoring expression levels and stability of His-tagged proteins
Immunoprecipitation and ELISA
Immunocytochemistry and immunohistochemistry
Features
Specifications
Detection Secondary antibody required
Epitope detected RGSHHHH
Sensitivity in dot blots -chemiluminescent substrate- 10 pg
Sensitivity in dot blots -chromogenic substrate- 0.5 ng
Sensitivity in western blots - Chemiluminescent substrate 50 pg
Sensitivity in western blots -chromogenic substrate- 2 ng
Substrate for blot detection Dependent on secondary antibody
Substrates for assay procedure Dependent on secondary antibody
Tag His tag

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Handbooks & Protocols (1)
For Anti·His Antibodies, Anti·His HRP Conjugates, Penta·His™ Alexa Fluor® Conjugates, Penta·His Biotin Conjugate, Ni-NTA Conjugates, Tag·100™ Antibody, Streptavidin–R-PE, 6xHis Protein Ladder, Ni-NTA HisSorb™ Strips and Plates 
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Kit Handbooks (1)
For Anti·His Antibodies, Anti·His HRP Conjugates, Penta·His™ Alexa Fluor® Conjugates, Penta·His Biotin Conjugate, Ni-NTA Conjugates, Tag·100™ Antibody, Streptavidin–R-PE, 6xHis Protein Ladder, Ni-NTA HisSorb™ Strips and Plates 
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