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Penta·His HRP Conjugate Kit

For sensitive, direct detection of His-tagged proteins

  • High-sensitivity and high-speed detection of His-tagged proteins
  • No need for secondary antibody
  • Complete kit with optimized blocking reagent

The Penta·His HRP Conjugate Kit is for sensitive detection of recombinant proteins carrying His tags without the need for secondary antibodies. It consists of Anti·His antibodies conjugated to horseradish peroxidase (HRP). In detection procedures, the Anti·His antibody binds to a protein's His tag and the complex is localized using chemiluminescent or chromogenic substrates that react with HRP.

Cat No./ID: 34460
Penta·His HRP Conjugate Kit
$515.00
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125 μl Penta·His HRP Conjugate, 5 g Blocking Reagent, 50 ml Blocking Reagent Buffer (10x concentrate)
The Penta·His HRP Conjugate Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Performance
Performance of Penta·His HRP Conjugate Kit
PropertyValue
Detection: N-terminal, C-terminal, and internal 6xHis tags
Sensitivity in dot blots:* 10 pg
Sensitivity in western blots:* 50 pg
Antibody Isotype: Mouse IgG1
Cross-reactivity: Negligible with crude E. coli, yeast, mammalian, or insect cell lysates
Form: Stabilized stock solution
Working solution: 125–250 ml
* Detection using chemiluminescent substrate. Sensitivity may vary with different proteins.
Principle
The Penta·His HRP Conjugate Kit consists of the QIAexpress Anti·His Antibodies chemically coupled to the reporter enzyme horseradish peroxidase (HRP). It can be used for highly specific and sensitive, direct detection of any recombinant protein that has an accessible His tag. The Anti·His HRP Conjugate Kits eliminate the need for secondary antibodies, saving time in immunodetection procedures.
Procedure
The QIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.
Applications

The Penta·His HRP Conjugate Kit can be used with chemiluminescent or chromogenic detection substrates in:

Western, dot, and colony blotting procedures
ELISA and other immunoassay procedures
Features
Specifications
Conjugated enzyme HRP
Detection Secondary antibody required
Epitope detected HHHHH
Substrate for blot detection 4C1N
Substrates for assay procedure OPD
Tag His tag

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Handbooks & Protocols (1)
For Anti·His Antibodies, Anti·His HRP Conjugates, Penta·His™ Alexa Fluor® Conjugates, Penta·His Biotin Conjugate, Ni-NTA Conjugates, Tag·100™ Antibody, Streptavidin–R-PE, 6xHis Protein Ladder, Ni-NTA HisSorb™ Strips and Plates 
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Kit Handbooks (1)
For Anti·His Antibodies, Anti·His HRP Conjugates, Penta·His™ Alexa Fluor® Conjugates, Penta·His Biotin Conjugate, Ni-NTA Conjugates, Tag·100™ Antibody, Streptavidin–R-PE, 6xHis Protein Ladder, Ni-NTA HisSorb™ Strips and Plates 
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