SureFIND Transcriptome PCR Arrays

For identification of molecular mechanisms that regulate gene expression

Ready to use cDNA panels
Analyze 90 specific cDNAs (transcriptomes) in a single PCR run
Discover miRNA or transcription factors involved in gene regulation
Discover signaling pathways involved in gene regulation
Compatible with SYBR Green or probe-based qPCR assays

SureFIND Transcriptome PCR Arrays are ready-to-use cDNA panels derived from cells that are treated with miRNA mimics, siRNAs for transcription factors, or chemicals inhibiting specific signaling pathways. Simply screen the cDNA samples using a verified gene-specific qPCR assay to discover which treatments change the expression of the gene of interest.

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SureFIND Transcriptome PCR Arrays are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Identification of miRNA regulators of INPPL1 expression.

PCR reactions using primers for INPPL1 (SHIP2) and a normalization gene were performed using the SureFIND Cancer miRNA Transcriptome PCR Array. The SureFIND Cancer miRNA Transcriptome PCR Array contains cDNA samples derived from HeLa cells treated with 90 different cancer-associated miRNA mimics. The software identified miRNA-205, –193b, –138, –373, –29b, and –135b as negative regulators of INPPL1 expression.

SureFIND Transcriptome PCR protocol.

SureFIND Transcriptome PCR Arrays identify miRNA regulators of INPPL1 expression.

PCR reactions using primers for INPPL1 (SHIP2) and a normalization gene were performed using the SureFIND Cancer miRNA Transcriptome PCR Array. The SureFIND Cancer miRNA Transcriptome PCR Array contains cDNA samples derived from HeLa cells treated with 90 different cancer-associated miRNA mimics. The software identified miRNA-205, -193b, -138, -373, -29b, and -135b as negative regulators of INPPL1 expression.

Performance

SureFIND Transcriptome PCR Arrays can be used to determine gene expression regulation in cancer studies (see figure SureFIND Transcriptome PCR Arrays identify miRNA regulators of INPPL1 expression).

Principle

SureFIND Transcriptome PCR Arrays are innovative tools for identifying which miRNA, signaling pathway, or transcription factor regulates the expression of any gene.

In each well of a SureFIND Transcriptome PCR Array is a unique "transcriptome" or cDNA sample synthesized from cells treated with a unique miRNA mimic, chemical inhibitor, or siRNA. Using real-time PCR, researchers can identify the mechanisms of gene expression regulation. A change in the expression of the tested gene in a specific sample shows that the corresponding miRNA, signaling pathway activity, or transcription factor is a regulator of that gene.

Available arrays

Discover miRNAs that target a gene with cDNA synthesized from cells treated with miRNA mimetics:

Cancer-related miRNA (90 miRNAs)
Immunopathology-related miRNA (90 miRNAs)
Development and differentiation-related miRNAs (90 miRNAs)
Complete miRNome (~900 miRNAs)

Discover pathways that regulate a gene with cDNA synthesized from cells treated with 90 different chemical inhibitors:

Pathway inhibitors (90 chemical inhibitor treatments)

Find transcription factor that regulate a gene with cDNA synthesized from cells treated with siRNAs targeting transcription factors (TF)

Cancer-related transcription factors (90 TF knockdowns)
Expressed transcription factors (270 TF knockdowns)

Procedure

Using SureFIND miRNA Transcriptome PCR Arrays is as simple as setting up a real-time PCR experiment. Simply mix primers with the appropriate PCR master mix, aliquot equal volumes into each well of the plate, and run the plate in a real-time PCR instrument (see figure "SureFIND Transcriptome PCR protocol").

After running the PCR, the Excel-based data analysis template for SureFIND Transcriptome PCR Arrays performs the ∆∆C_T-based fold-change. The results are delivered in a tabular format and assists in hit selection.

Applications

SureFIND Transcriptome PCR Arrays can be used to discover the mechanisms that regulate the expression of any gene.