text.skipToContent text.skipToNavigation

miScript SYBR® Green PCR Kit

For miRNA detection by real-time PCR using the miScript PCR System

  • Sensitive and specific detection and quantification of miRNA
  • Quantification of hundreds of miRNAs from a single cDNA
  • Quantification of miRNA and mRNA from the same cDNA
  • Includes ROX dye for optional normalization of fluorescence

The miScript SYBR Green PCR Kit is an integral component of the miScript PCR System for miRNA detection and quantification. The kit is based on the proven technology of QuantiTect SYBR Green Kits. cDNA generated by the miScript II RT Kit is used as the template for real-time PCR with the miScript SYBR Green PCR Kit and either PCR arrays (miRNome or Pathway-Focused miScript miRNA PCR Arrays) or appropriate assays (miScript Primer Assays for miRNA or other noncoding RNA, miScript Precursor Assays for precursor miRNA, or QuantiTect Primer Assays for mRNA).

Cat No./ID: 218073
miScript SYBR Green PCR Kit (200)
$488.00
Add To Cart
For 200 reactions: QuantiTect SYBR Green PCR Master Mix, miScript Universal Primer
Cat No./ID: 218075
miScript SYBR Green PCR Kit (1000)
$1,967.00
Add To Cart
For 1000 reactions: QuantiTect SYBR Green PCR Master Mix, miScript Universal Primer
Cat No./ID: 218076
miScript SYBR Green PCR Kit (2000)
$2,620.00
Add To Cart
For 2000 reactions: QuantiTect SYBR Green PCR Master Mix, miScript Universal Primer
The miScript SYBR® Green PCR Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
0
miRNA expression in colorectal cancer.

cDNA was prepared from human colon adenocarcinoma total RNA and normal adjacent colon total RNA. miRNome profiling was performed using the Human miRNome miScript miRNA PCR Array. Fifty-six miRNAs were strongly downregulated and 50 were strongly upregulated in the adenocarcinoma tissue (red lines indicate ±4-fold change).

Performance

The miScript SYBR Green PCR Kit, together with the components of the miScript PCR System, ensures sensitive, specific miRNA detection and quantification. The miScript PCR System provides exceptional sensitivity and a broad dynamic range. Amplification efficiencies are consistently high, enabling accurate expression analysis of multiple miRNAs using the ∆∆CT method (see figure miRNA expression in colorectal cancer). The high performance of the miScript PCR System ensures highly reproducible results between technical and biological replicates at the first attempt.

Principle

The miScript PCR System enables sensitive, specific miRNA quantification and profiling using SYBR Green-based real-time PCR. The miScript PCR System covers all the steps involved in conversion of RNA to cDNA and subsequent real-time PCR detection of miRNAs.

The miScript PCR System comprises:

miScript II RT Kit — this kit enables simple, single-step cDNA synthesis. A single cDNA synthesis reaction can be used for detection of hundreds of miRNAs. The dual buffer system meets the distinctive needs of miRNA quantification using real-time PCR.
miScript PreAMP PCR Kit and Primer Mixes — the kit and primer mixes enable unbiased preamplification of limited miRNA amounts, allowing subsequent miRNA profiling.
miScript SYBR Green PCR Kit — this kit includes QuantiTect SYBR Green PCR Master Mix and the miScript Universal Primer, a reverse primer that allows detection of miRNAs in combination with a miScript Primer Assay or miScript miRNA PCR Array.
miScript Primer Assay or miScript Precursor Assay — miScript Primer Assays are miRNA-specific forward primers designed to detect mature miRNAs. miScript Precursor Assays consist of forward and reverse primers targeting precursor miRNA stem-loop sequences. Assays are designed using the most up-to-date sequence information from miRBase.
miScript miRNA PCR Array — miRNome or Pathway-Focused miScript miRNA PCR Arrays are preformatted, single-use PCR arrays for rapid profiling of mature miRNAs.
miScript miRNA PCR Array data analysis tool — this complimentary, Web-based tool simplifies the ΔΔCT method of relative quantification for miScript miRNA PCR Arrays.
Real-time PCR detection of miRNA

The miScript SYBR Green PCR Kit includes QuantiTect SYBR Green PCR Master Mix and the miScript Universal Primer, which is specific for the universal tag sequence added to the 5' end of the cDNA during reverse transcription. A single cDNA synthesis reaction is sufficient to interrogate hundreds of miRNAs. cDNA prepared using miScript HiSpec Buffer can be used for mature miRNA quantification with miScript miRNA PCR Arrays or miScript Primer Assays. cDNA prepared using miScript HiFlex Buffer can be used for detection of different RNA species with appropriate primers in the real-time PCR step: miScript Primer Assays for miRNA or other noncoding RNA, miScript Precursor Assays for precursor miRNA, or QuantiTect Primer Assays for mRNA. 
 

Procedure

Reverse transcription is performed using the miScript II RT Kit. The resultant cDNA is used as a template for real-time PCR performed with the miScript SYBR Green PCR Kit, together with appropriate assays or arrays. This makes miRNA quantification and profiling a straightforward procedure, accessible to any lab with a real-time PCR cycler.

Applications

The miScript SYBR Green PCR Kit is used as part of the miScript PCR System for:

Mature miRNA quantification and profiling
miRNA and mRNA detection in parallel
Mature miRNA and precursor miRNA detection in parallel
snoRNA and other noncoding RNA detection

You are not authorized to download the resource

Kit Handbooks (5)

Show details
For real-time PCR analysis of microRNA using SYBR Green detection
Show details
For SYBR Green-based, real-time PCR profiling of microRNAs using pathway-focused arrays, HC arrays, and miRNome arrays
Show details
SYBR Green検出を用いたmicroRNAのリアルタイムPCR解析
Show details

Show details
fragment fix placeholder

Customers who bought these products also bought

  • Cat No./ID: 331452
    /qiagenstorefront/ca//pcr/real-time-pcr-enzymes-and-kits/qpcr/miscript-preamp-pcr-kit/

    miScript PreAMP PCR Kit (60)

    For 60 preamplification reactions: 5x miScript PreAMP Buffer, HotStarTaq DNA Polymerase (2 U/µl), miScript PreAMP Universal Primer, miR-16 miScript Primer Assay, SNORD95 miScript Primer Assay, miRNA reverse transcription control (miRTC), C. elegans miR-39 miScript Primer Assay, RNase-Free Water
    $1,289.00
  • Cat No./ID: 218161
    /qiagenstorefront/ca//pcr/real-time-pcr-enzymes-and-kits/two-step-qrt-pcr/miscript-ii-rt-kit/

    miScript II RT Kit (50)

    For 50 cDNA synthesis reactions: miScript Reverse Transcriptase Mix, 10x miScript Nucleics Mix, 5x miScript HiSpec Buffer, 5x miScript HiFlex Buffer, RNase-Free Water
    $549.00
  • Cat No./ID: 218073
    /qiagenstorefront/ca//pcr/real-time-pcr-enzymes-and-kits/two-step-qrt-pcr/miscript-sybr-green-pcr-kit/

    miScript SYBR Green PCR Kit (200)

    For 200 reactions: QuantiTect SYBR Green PCR Master Mix, miScript Universal Primer
    $488.00
  • Cat No./ID: 217184
    /qiagenstorefront/ca//sample-technologies/rna/mirneasy-serumplasma-kit/

    miRNeasy Serum/Plasma Kit (50)

    For 50 total RNA preps: 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, Ce_miR-39_1 miScript Primer Assay, RNase-free Reagents and Buffers
    $475.00
  • Cat No./ID: 217004
    /qiagenstorefront/ca//sample-technologies/rna/mirneasy-mini-kit/

    miRNeasy Mini Kit (50)

    For 50 preps: 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-Free Reagents and Buffers
    $380.00
  • Cat No./ID: 74104
    /qiagenstorefront/ca//sample-technologies/rna/rneasy-mini-kit/

    RNeasy Mini Kit (50)

    50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
    $361.00
  • Cat No./ID: 218160
    /qiagenstorefront/ca//pcr/real-time-pcr-enzymes-and-kits/two-step-qrt-pcr/miscript-ii-rt-kit/

    miScript II RT Kit (12)

    For 12 cDNA synthesis reactions: miScript Reverse Transcriptase Mix, 10x miScript Nucleics Mix, 5x miScript HiSpec Buffer, 5x miScript HiFlex Buffer, RNase-Free Water
    $145.00
  • Cat No./ID: 79254
    /qiagenstorefront/ca//lab-basics/enzymes/rnase-free-dnase-set/

    RNase-Free DNase Set (50)

    1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps
    $125.00
  • Cat No./ID: 218300
    /qiagenstorefront/ca//pcr/miscript-pcr-controls/

    miScript Primer Assay (100)

    10x miScript Primer Assay (contains one miRNA-specific primer)
  • Cat No./ID: 219610
    /qiagenstorefront/ca//sample-technologies/rna/mirneasy-serumplasma-kit/

    miRNeasy Serum/Plasma Spike-In Control

    10 pmol lyophilized C. elegans miR-39 miRNA mimic
    $49.20