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GeneRead DNA QuantiMIZE Kits

For quantification and qualification of amplifiable DNA prior to NGS
  • Provides 2 qPCR assays dispensed onto arrays or in individual tubes
  • Quantifies and qualifies DNA that is amplifiable by PCR
  • Guides optimization of targeted enrichment conditions
  • Requires no serial dilutions
The GeneRead DNA QuantiMIZE System is a qPCR-based approach to determine quantity and quality of DNA that is amenable to amplification by targeted enrichment prior to NGS. The system has been developed for discovery, translational, and clinical researchers who need a cost-effective way to determine the optimum conditions for targeted enrichment of DNA isolated from biological samples, including FFPE. In contrast to other approaches, the system improves the likelihood of a successful NGS run by not only quantifying amplifiable DNA, but also optimizing the target enrichment conditions. 

Please note: GeneRead DNA QuantiMIZE Array Kit (product number 180642) and GeneRead DNA QuantiMIZE Assay Kit (product number 180654) will be phased out by the end of 2016. Please use QIAseq DNA QuantiMIZE Array Kit (product number 333404) and QIAseq DNA QuantiMIZE Assay Kit (product number 333414instead.
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2 arrays in Formats A, C, D, or F; or 2 discs in Format R; or 1 array in Format E or G; QuantiMIZE Control gDNA (50 μl; GeneRead qPCR SYBR Green Mastermix (2 x 1.35 ml)
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180642 NGQA-002 varies
Assay 100 and Assay 200 for 400 x 25 μl reactions (400 μl) each; QuantiMIZE Control gDNA (50 μl); RNase- and DNase-free water (1 ml x 4); GeneRead qPCR SYBR Green Mastermix (1.35 ml x 4)
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180654 NGQC-100Y varies
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GeneRead DNA QuantiMIZE Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


Performance
Quantification and qualification of amplifiable DNA by the GeneRead DNA QuantiMIZE System rescues low-quality FFPE samples for NGS analysis. Use of the GeneRead DNA QuantiMIZE System resulted in high-quality libraries that were succesfully sequenced on a MiSeq (see Successful quantification and qualification of DNA by the GeneRead DNA QuantiMIZE system). 
Principle
GeneRead DNA QuantiMIZE System utilizes 2 qPCR assays that interrogate 20 genomic loci to determine the amounts of amplifiable DNA in biological samples. The assays, Assay 100 and Assay 200, generate 100 and 200 bp amplicons, respectively. QuantiMIZE Kits use the CT values of Assay 100 and Assay 200 for both the Control samples and the Case samples to quantify and qualify DNA (see Principle of the GeneRead DNA QuantiMIZE System).  
Procedure
Genomic DNA extracted from biological samples is mixed with the supplied mastermix. This mixture is then aliquoted onto arrays, if the array kits are used, or mixed with Assay 100 and Assay 200 before being aliquoted onto arrays, if the assay kits are used. PCR is performed and the raw CT values are exported to a web-based data analysis module, which will quantify and qualify DNA as well as provide guidance on DNA amounts and PCR cycles needed to optimize targeted enrichment conditions. 
Applications
GeneRead DNA QuantiMIZE Kits can be used to quantify and qualify any DNA sample. They are especially beneficial for low‑quality DNA samples, such as FFPE (see Robust performance with FFPE samples). Additionally, the kits can be used to optimize the conditions needed for successful targeted enrichment of genomic regions from any DNA sample, including low-quantity DNA samples, such as FFPE, and low-quantity DNA samples, such as fine-needle aspirates.

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