For studies on miRNA function and gene regulation using miRNA inhibition

  • Ready-to-transfect inhibitors bind and inhibit miRNAs
  • Available for all human, mouse, and rat miRNAs in miRBase
  • Highly flexible formats, scales, and grades
  • Custom design available for miRNAs not listed in miRBase

miScript miRNA Inhibitors are chemically synthesized, single-stranded, modified RNAs which specifically inhibit endogenous miRNA function after transfection into cells. miScript miRNA Inhibitors are available at cell-culture grade (>90% purity) or animal grade (HPLC purified). miScript miRNA Inhibitors are also available phosphorothioate modified at the 20 nmol scale. For flexible screening, miScript miRNA Inhibitor Plates enable researchers to order any predesigned or custom-designed miScript miRNA Inhibitors in their choice of layout in 96-well or 384-well plates. miScript miRNA Mimics can be used for similar experiments.

Search in GeneGlobe
You can search for the following terms:
  • Entrez Gene IDs (e.g., 835)
  • RefSeq IDs (e.g., NM_032983, NP_116765)
  • Gene symbols (e.g., CASP2)
  • Cat. no. (e.g., SI00299551, QT01342509)
  • Sanger ID or Accession (e.g., hsa-let-7b, MI0000063)
  • CpG loci identification numbers (CG#) (e.g., CG17753661)

Do not enter species information in the Search box. Use the drop-down list to select your species of interest.
When searching for miRNAs, do not omit the hyphens. Use hyphens or spaces (e.g., search for hsa-let-7b or hsa let 7b. Do not search for hsalet7b).

Advanced search settings



Species:
Products
Product Product no. Cat. no. List price:
 
 
show details
    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
Product Product no. Cat. no. List price:
miScript miRNA Inhibitor
1 nmol (cell-culture grade), 5 nmol (cell-culture grade), or 20 nmol (animal grade, option of phosphorothioate modification) miScript miRNA Inhibitor, provided in tubes
219300 Varies
Inquire
Enter your targets in search box above.

Can’t order online or need plate formats? Order Forms and Plate Orders

miScript miRNA Inhibitors are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


  • Main Image Navi
miRNA Inhibitor effective after 96 hours.|miScript miRNA Inhibitor counteracts miRNA-induced silencing.|Transfection of miRNA inhibitor decreases endogenous miRNA level.|
HeLa S3 cells (6 x 104 cells/well) were transfected using HiPerFect Transfection Reagent with miR-15a Inhibitor, miR-16 Inhibitor, miR-21 Inhibitor, miR-25 Inhibitor, or an Inhibitor with a scrambled sequence (negative control). At the time points indicated, luciferase reporter constructs with the appropriate miRNA binding sites were transfected. A reporter construct that is not regulated by any miRNA was used as positive control. Twenty-four hours later, luciferase activity was measured.|HeLa S3 cells express miR-16 at high levels and do not express miR-1 (see Ref. 1). In a cotransfection experiment using HiPerFect Transfection Reagent, HeLa S3 cells (6 x 104 cells/well) were cotransfected with a luciferase reporter construct with an miR-16 binding site in the 3' UTR together with miR-16 Inhibitor. miR-16 Inhibitor was used at varying concentrations in the experiment to evaluate the optimal inhibitor concentration required to see the inhibitory effect. Alternatively, cells were transfected with miR-1 Inhibitor alone as a control. A luciferase construct without an miRNA binding site (WT) was transfected as a positive control. An increase in luciferase expression following transfection of the Inhibitor indicated that the miR-16 Inhibitor prevented endogenous miR-16 from downregulating the reporter gene.

1. Lagos-Quintana, M. et al. (2001) Identification of novel genes coding for small expressed RNAs. Science 294, 853. |HeLa S3 cells (6 x 104 cells/well) were transfected using HiPerFect Transfection Reagent with miScript miR-16 Inhibitor or a negative control. Endogenous miR-16 levels were quantified by real-time PCR using the miScript System. U6B was used as the reference RNA for data normalization and miR-16 levels were expressed as 2-ΔΔCT values relative to the miR-16 levels in untransfected cells. [A] Transfection of miScript miR-16 Inhibitor strongly reduced the amount of detectable endogenous miR-16. [B] The amplification plots indicated a significant decrease in the level of detectable miR-16 as a result of miR-16 Inhibitor transfection. Dissociation curve analysis (inset) reflects the specificity of the real-time PCR detection using the miScript System.|

Performance
miScript miRNA Inhibitors have been designed and modified to ensure efficient inhibition of endogenous miRNA function (see figure miScript miRNA Inhibitor counteracts miRNA-induced silencing). Real-time PCR analysis detected a significant decrease in miRNA level after transfection of miScript miRNA Inhibitor (see figure "Transfection of miRNA inhibitor decreases endogenous miRNA level"). Time-course experiments indicate that miScript miRNA Inhibitors are effective for at least 96 hours following transfection (see figure "miRNA inhibitor effective after 96 hours").
Principle

Transfection of inhibitors, followed by downstream gene expression analysis or phenotypic analysis, is performed to elucidate the targets and roles of particular miRNAs. These experiments enable study of the biological effects of misregulation of individual miRNAs as well as confirmation of specific genes as targets of individual miRNAs.

Increased gene expression after transfection of inhibitor provides evidence that the miRNA under study is involved in regulation of that gene. Similar studies can be performed using miScript miRNA Mimics. Typically, such experiments involve transfection of mimic or inhibitor, or alternatively cotransfection with a vector construct which carries the miRNA-binding site fused to a reporter gene. Downstream analysis can include reporter assays, real-time PCR, microarray analysis, or protein analysis.

Procedure

Highly flexible solutions offered by miScript miRNA Inhibitors are available for every human, mouse, rat, and virus miRNA in the current version of the microRNA database, miRBase. QIAGEN's GeneGlobe database is continually updated to ensure that mimic and inhibitor designs match the most up-to-date version of miRBase. This ensures that you are always working with the most accurate and current miRNA sequence information possible. If the miRNA of interest is not available in miRBase, an inhibitor for your human, mouse, rat, or virus miRNA can be custom synthesized by visiting QIAGEN's custom miScript product design page.

Flexible formats enable either low-throughput analysis using small numbers of inhibitors or high-throughput screening using hundreds of inhibitors. miScript miRNA Inhibitors are available at cell-culture-grade (>90% purity provided by proprietary synthesis and purification processes). For in vivo applications, HPLC-purified, animal-grade miScript miRNA Inhibitors can be ordered at the 20 nmol scale (provided in tubes). miScript miRNA Inhibitors are also available phosphorothioate modified at the 20 nmol scale for in vivo applications. Phosphorothioate modification protects the inhibitor from exonuclease activity, increasesing the stability of the molecule. miScript miRNA Inhibitors can easily be ordered at GeneGlobe. For medium- to high-throughput applications, 96-well plates, or 384-well plates are available to suit your experimental requirements.

Applications
miRNA research
Gene regulation research
Feature
Specifications
Design Predesigned and custom design avaliable
Format Single tubes, 96-well & 384 well plates
Modification Yes, optional with 20 nmol: phosphorothiate modified for in vivo studies
Scale or yield 1 nmol, 5 nmol, 20 nmol
Sequence provided No
Species Human, mouse, rat

You are not authorized to download the resource