For multiplex amplification of 13 CODIS core loci, plus D2S1338, D19S433, and Amelogenin

  • PCR reaction speed of around 90 minutes
  • Highly sensitive and extremely inhibitor resistant
  • High-quality results due to improved signal-to-noise ratio
  • Straightforward protocols with ready-to-use Fast Reaction Mix
  • Simultaneous amplification of 16 loci

The Investigator IDplex Plus Kit is a multiplex application for human identification covering the 13 CODIS (Combined DNA Index System) core loci, D2S1338, D19S433, and Amelogenin. The kit utilizes QIAGEN's fast-cycling technology, enabling amplification in just 90 minutes. Highly sensitive with rapid and reliable results from trace DNA, the Investigator IDplex Plus Kit is suitable for all forensic applications and paternity testing. The genetic analyzer is calibrated with BT5 Matrix, an optimized dye technology.

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Product Cat. no. List price:
Investigator IDplex Plus Kit (100)
Primer mix, Fast Reaction Mix including HotStarTaq Plus DNA Polymerase, Control DNA, allelic ladder IDplex Plus, DNA size standard 550 (BTO), and nuclease-free water
381625
$1,813.00

The Investigator IDplex Plus Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease. This product is not available in all countries.


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Performance

The Investigator IDplex Plus Kit utilizes fast-cycling technology to provide reliable results in approximately 90 minutes, which is significantly faster than kits from other suppliers.

The novel Fast Reaction Mix is based on proven QIAGEN multiplex PCR technology that has been further optimized to increase inhibitor tolerance. Assay performance tested in the presence of increasing concentrations of 6 types of inhibitor (humic acid, hematin, tannic acid, indigo carmine, calcium, and collagen) shows high sensitivity and superior inhibitor resistance. This enables the generation of reliable, high-quality results.

The Investigator IDplex Plus Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode) and the Applied Biosystems 3500 Genetic Analyzer.

The recommended amount of DNA under standard conditions is 0.5 ng. Internal validations demonstrated robust and balanced results with 0.2-2 ng DNA, and reliable results with <0.1 ng DNA.

Principle

The Investigator IDplex Plus Kit uses QIAGEN's fast-cycling PCR technology for the rapid, simultaneous amplification of the 13 CODIS (Combined DNA Index System) core loci, D2S1338, D19S433, and Amelogenin. Carefully designed primers prevent overlap of loci alleles, thereby minimizing the likelihood of misinterpretation.

Procedure

The 15 polymorphic STR markers (D2S1338, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D19S433, D21S11, CSF1PO, FGA, TH01, TPOX, and vWA), and the gender-specific Amelogenin are amplified simultaneously in a single PCR  and labeled with one of the dyes listed in the table "Investigator IDplex Plus Kit dyes and corresponding markers". 

The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as QIAGEN's Investigator IDproof or IDproof Mixture Software. See the table "Technical specifications" for more information.

Investigator IDplex Plus Kit dyes and corresponding markers
DyeMarker
6-FAM Amelogenin TH01 D3S1358 vWA D21S11
BTG TPOX D7S820 D19S433 D5S818 D2S1338
BTY D16S539 CSF1PO D13S317 FGA
BTR D18S51 D8S1179 
Technical specifications
Volume per amplification reaction 25 μl
Matrix BT5
Fluorescence labels 6-FAM, BTG, BTY, BTR, BTO
Genetic analyzers ABI PRISM 310, ABI PRISM 3100/3100-Avant, Applied Biosystems 3130/3130xl, or Applied Biosystems 3500/3500xL Genetic Analyzers
Software for analysis QIAGEN Investigator IDproof or Investigator IDproof Mixture Software, or Applied Biosystems GeneMapper ID, GeneMapper ID-X, or Genotyper Software
Applications

The Investigator IDplex Plus Kit is for rapid and reliable generation of DNA profiles from blood, buccal swabs, and forensic stains, and it is highly suited for:

Criminal database and forensic casework samples
Paternity testing
Population genetics studies
Other human identity testing applications

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