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ipsogen MN1 ProfileQuant Kit

For quantitative detection of MN1 gene transcripts using real-time PCR
  • Sensitive quantitative assay
  • Accurate quantification relative to ABL control gene expression
  • Optimal quality control and ISO 13485 certification
  • Easy workflow with ready-to-use solutions
The ipsogen MN1 ProfileQuant Kit is intended for research use only and is not for use in diagnostic procedures. The kit is for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The ipsogen MN1 ProfileQuant Kit provides reagents optimized for reliable and sensitive detection and quantification of MN1 gene transcripts in human cells, relative to ABL control gene expression.
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
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Sensitive detection of MN1 and ABL standards.
Detection of [A] MN1 standards (P1 to P5) with 101, 102, 103, 105, and 106 copies/5 μl and [B] ABL standards (C1, C2, C3) with 103, 104, and 105 copies/5 μl.
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MN1 gene transcript.
Performance
To ensure highest sensitivity, the ipsogen MN1 ProfileQuant Kit has been optimized to detect ABL and MN1 gene transcripts over a wide dynamic range (see figure "Sensitive detection of ABL and MN1 standards").
Principle
The ipsogen MN1 ProfileQuant Kit is a ready-to-use kit for detection of MN1 gene transcripts using real-time PCR. The kit is based on the amplification and detection of specific MN1 gene transcripts, relative to ABL control gene expression in total RNA (see figures "MN1 gene transcript" and "ABL control gene transcript", and provides high levels of specificity, sensitivity, and reproducibility.
Each ipsogen MN1 ProfileQuant Kit provides 3 standard dilutions for ABL and 5 standard dilutions for the MN1 gene.
Procedure
The first step is reverse transcription of total RNA in a sample into cDNA. The second step is the amplification of cDNA by real-time PCR. Using the ipsogen MN1 ProfileQuant Kit allows detection and quantification of MN1 and ABL gene transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.
Applications
The ipsogen MN1 ProfileQuant Kit enables sensitive and reliable detection and quantification of MN1 gene transcripts relative to ABL control gene expression for research use only.
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