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ipsogen BCR-ABL1 mbcr Kit

For quantitative detection of BCR-ABL mbcr e1a2 transcripts using real-time PCR
  • Accurate and sensitive quantitative assay
  • EAC standardized assay for reproducible results
  • Easy workflow with ready-to-use solutions
 
The ipsogen BCR-ABL1 mbcr Kit is intended for research use only and is not for use in diagnostic procedures. The kit is for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The kit provides reagents optimized for reliable and sensitive detection and quantification of BCR-ABL mbcr p190 e1a2 transcripts, relative to ABL control gene expression, in total RNA.
Cat No./ID: 670013
ipsogen BCR-ABL1 mbcr Kit (24)
For 24 reactions: ABL Control Gene Standards, BCR-ABL mbcr Fusion Gene Standards, Primers and Probe Mix ABL, Primers and Probe Mix BCR-ABL mbcr Fusion Gene
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
0
BCR-ABL mbcr fusion gene transcript.
2
Accurate detection of BCR-ABL Mbcr standards.
BCR-ABL mbcr standards (F1–F5): 101, 102, 103, 105, and 106 copies/5 µl.
Performance
To ensure highest sensitivity, the ipsogen BCR-ABL1 mbcr Kit has been optimized to detect BCR-ABL mbcr p190 e1a2 and ABL transcripts and uses prediluted plasmid standards and primers and probe mixes (see figures Accurate detection of BCR-ABL mbcr plasmid standards and Reliable detection of ABL plasmid standards).
Principle
The ipsogen BCR-ABL1 mbcr Kit is a ready-to-use kit for the detection of BCR-ABL mbcr p190 e1a2 fusion gene transcripts using real-time PCR. The kit is based on the amplification and detection of specific BCR-ABL mbcr p190 e1a2 transcripts, relative to ABL control gene expression, in total RNA (see figures BCR-ABL mbcr fusion gene transcript and ABL control gene transcript). The kit provides high levels of specificity, sensitivity, and reproducibility. The ipsogen BCR-ABL1 mbcr Kit provides 3 standard dilutions for ABL and 5 standard dilutions for BCR-ABL mbcr. Use of the standards enables accurate quantification of transcripts. The technology used for quantification of BCR-ABL mbcr has been standardized according to EAC (Europe Against Cancer) recommendations and the ipsogen BCR-ABL1 mbcr Kit uses this validated technology to calibrate results.
Procedure
The first step is reverse transcription of total RNA in a sample into cDNA. The second step is the amplification of cDNA by real-time PCR. Using the ipsogen BCR-ABL1 mbcr Kit allows detection and quantification of BCR-ABL mbcr p190 e1a2 and ABL transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.
Applications
The ipsogen BCR-ABL1 mbcr Kit enable sensitive and reliable detection and quantification of BCR-ABL mbcr p190 e1a2 transcripts, relative to ABL control gene expression, for research use only.
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