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EGFR RGQ PCR Kit

For sensitive detection of 29 somatic mutations in the EGFR oncogene in less than 4 hours

  • Robust test that can detect low levels of mutant DNA
  • Ready-to-use system with simple workflow
  • Selective and sensitive due to ARMS and Scorpions technologies

The EGFR RGQ PCR Kit is a research use only assay for detection of specific mutations, insertions, and deletions in the EGFR gene using real-time PCR on the Rotor-Gene Q 5plex HRM instrument. The kit provides all necessary reagents optimized for rapid and sensitive detection of a low percentage of mutant DNA in a background of wild-type genomic DNA. ARMS PCR technology combined with the Scorpions detection technology provides high sensitivity and specificity for the detection of specific mutations.

Cat No./ID: 870101
EGFR RGQ PCR Kit (24)
For 24 reactions: 1 Control Assay, 7 Mutation Assays, Mixed Standard, Taq DNA Polymerase
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Principle

The EGFR RGQ PCR Kit enables detection of 29 somatic mutations in the EGFR gene. The EGFR gene encodes the epidermal growth factor receptor (EGFR) protein. Mutations in the tyrosine kinase domain of the EGFR gene can enable tumor growth and progression. EGFR mutations are found in approximately 10% of non-small cell lung cancer incidences in the US and 35% in East Asia. Additionally, EGFR mutations are found in 6% of brain tumors.

The EGFR RGQ PCR Kit enables detection of 29 somatic mutations in the EGFR gene including:

19 deletions in exon 19
T790M 
L858R
L861Q
G719X
S768I

3 insertions in exon 20

The kit utilizes two technologies — ARMS  (Amplification Refractory Mutation System) and Scorpions — for detection of EGFR mutations using real-time PCR on the Rotor-Gene Q 5plex HRM instrument.

ARMS

Allele- or mutation-specific amplification is achieved by ARMS. Taq DNA polymerase is effective at distinguishing between a match and a mismatch at the 3' end of a PCR primer. Specific mutated sequences are selectively amplified, even in samples where the majority of the sequences do not carry the mutation. When the primer is fully matched, the amplification proceeds with full efficiency. When the 3' base is mismatched, only low-level background amplification occurs.

Scorpions

Detection of amplification is performed using Scorpions. Scorpions are bifunctional molecules containing a PCR primer covalently linked to a probe. The fluorophore in this probe interacts with a quencher, also incorporated into the probe, that reduces fluorescence. When the probe binds to the amplicon during PCR, the fluorophore and quencher become separated. This leads to an increase in fluorescence from the reaction tube.

Procedure

The EGFR RGQ PCR Kit uses a two-step procedure. The first step is performance of the control assay to assess the total DNA in a sample. The second step is to complete the mutation assay for the presence or absence of mutated DNA.

Applications

The EGFR RGQ PCR Kit enables detection of 29 somatic mutations in the EGFR gene including:

T790M, G719X, S768I
L858R, L861Q
3 insertions in exon 20
19 deletions in exon 19

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Kit Handbooks (2)

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Rotor-Gene Q 5plex HRM 装置を用いた、がん関連遺伝子、EGFR における29 種類の体細胞変異検出用
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Safety Data Sheets (1)
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