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artus WNV LC RT-PCR Kit RUO

For quantitative detection of West Nile virus RNA using real-time PCR
  • High reliability using the internal control
  • Sensitive detection of as few as 2.4 copies/µl
  • Accurate quantitation using the 4 standards supplied
The artus WNV LC PCR Kit is a ready-to-use molecular detection kit for real-time PCR. The kit provides all necessary reagents optimized for reliable West Nile virus RNA detection and quantitation.
Cat No./ID: 4509003
artus WNV LC RT-PCR Kit (24) RUO
For 24 reactions on the LightCycler 1.1/1.2/1.5 Instruments: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Cat No./ID: 4509005
artus WNV LC RT-PCR Kit (96) RUO
For 96 reactions on the LightCycler 1.1/1.2/1.5 Instruments: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Performance

To ensure sensitivity, the artus WNV LC RT-PCR Kit has been optimized to detect low numbers of West Nile virus RNA. The analytical sensitivity of the artus WNV LC RT-PCR Kit is 2.4 copies/µl in the PCR on the LightCycler 1.1/1.2/1.5 Instruments.

Performance of artus WNV LC RT-PCR Kit
Kitartus WNV LC RT-PCR Kit
Sample types Serum, plasma, or cerebral spinal fluid (CSF)
Analytical sensitivity 2.4 copies/µl in the PCR

Principle

The artus WNV LC RT-PCR Kit is based on the amplification and simultaneous detection of a specific region of the West Nile virus genome using real-time PCR. The kit provides high levels of specificity and sensitivity.

Each artus WNV LC RT-PCR Kit provides 4 quantitation standards (see figure "Reliable quantitation of WNV load"). Use of the standards enables accurate quantitation of viral load. In addition, the kit contains a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical WNV RT-PCR is not reduced.

Specifications of artus WNV LC RT-PCR Kit
Kitartus WNV LC RT-PCR Kit
Sample types Serum, plasma, or cerebral spinal fluid (CSF)
Amplicon 72 bp region of the West Nile virus genome

Procedure

The artus WNV LC RT-PCR Kit provides all necessary reagents optimized for reliable West Nile virus RNA detection and quantitation. Simply add template RNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

For RNA purification, we recommend using the QIAamp Viral RNA Mini Kit.

Applications
The artus WNV LC RT-PCR Kit enables rapid and sensitive detection of West Nile virus RNA from serum, plasma, and cerebral spinal fluid (CSF) with accurate quantification. The kit is available for use on the LightCycler 1.1/1.2/1.5 Instruments.
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