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artus SARS RG RT-PCR Kit RUO

For quantitative detection of SARS coronavirus RNA using real-time PCR
  • High reliability using the internal control
  • Sensitive detection of as few as 0.5 copies/µl
  • Accurate quantitation using the 4 standards supplied
The artus SARS RG RT-PCR Kit is a ready-to-use molecular detection kit for real-time PCR. The kit provides all necessary reagents optimized for reliable SARS coronavirus RNA detection and quantitation.
Cat No./ID: 4511203
artus SARS RG RT-PCR Kit (24) RUO
For 24 reactions on the Rotor-Gene 3000: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
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artus SARS RG RT-PCR Kit RUO.
Performance

To ensure sensitivity, the artus SARS RG RT-PCR Kit has been optimized to detect low numbers of SARS coronavirus RNA. The analytical sensitivity of the artus SARS RG RT-PCR Kit is 0.5 copies/µl in the PCR on the Rotor-Gene 3000.

Performance of artus SARS RG RT-PCR Kit
Kitartus SARS RG RT-PCR Kit
Sample types Sputum, serum, bronchoalveolar lavage (BAL), nasopharyngeal wash, swabs, or lung tissue
Analytical sensitivity 0.5 copies/µl in the PCR

Principle

The artus SARS RG RT-PCR Kit is based on the amplification and simultaneous detection of a specific region of the SARS coronavirus genome using real-time PCR. The kit provides high levels of specificity and sensitivity.

Each artus SARS RG RT-PCR Kit provides 4 quantitation standards (see figure "Reliable quantitation of SARS coronavirus load"). Use of the standards enables accurate quantitation of viral load. In addition, the kit contains a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical SARS coronavirus RT-PCR is not reduced.

Specifications of artus SARS RG RT-PCR Kit
Kitartus SARS RG RT-PCR Kit
Sample types Sputum, serum, bronchoalveolar lavage (BAL), nasopharyngeal wash, swabs, or lung tissue
Amplicon 92 bp region of the SARS coronavirus genome

Procedure

The artus SARS RG RT-PCR Kit provides all necessary reagents optimized for reliable SARS coronavirus RNA detection and quantitation. Simply add template RNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

For RNA purification, we recommend using the QIAamp Viral RNA Mini Kit (for sputum, serum, bronchoalveolar lavage [BAL], nasopharyngeal wash, or swabs) or the RNeasy Mini Kit (for lung tissue).

Applications
The artus SARS RG RT-PCR Kit enables rapid and sensitive detection of SARS RNA from sputum, serum, bronchoalveolar lavage (BAL), nasopharyngeal wash, swabs, and lung tissue with accurate quantification. The kit is available for use on the Rotor-Gene 3000.

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