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artus M. tuberculosis PCR Kits RUO

For quantitative detection of DNA of members of the M. tuberculosis complex using real-time PCR
  • High reliability using the internal control
  • Sensitive detection of as few as 0.23 copies/µl (RG kit)
  • Accurate quantitation using the 4 standards supplied
artus M. tuberculosis PCR Kits are ready-to-use molecular detection kits for real-time PCR. The kits provide all necessary reagents optimized for reliable detection and quantitation of DNA from all members of the M. tuberculosis complex (M. tuberculosis, M. africanum, M. bovis, M. bovis BCG, M. microti, and M. pinnipedii).
Cat No./ID: 4555003
artus M.tuberculosis LC PCR Kit (24) RUO
For 24 reactions on the LightCycler 1.1/1.2/1.5/2.0 Instruments: Master, Mg Solution, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Cat No./ID: 4555205
artus M.tuberculosis RG PCR Kit (96) RUO
For 96 reactions on Rotor-Gene Q instruments: Master, Mg Solution, 4 Quantitation Standards, Internal Control, Water (PCR grade)
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Performance

To ensure sensitivity, artus M. tuberculosis PCR Kits have been optimized to detect low numbers of mycobacterial DNA. The analytical sensitivity of the artus M. tuberculosis RG PCR Kit is 0.23 copies/µl in the PCR on the Rotor-Gene Q/6000 instrument.

Performance of artus M. tuberculosis RG PCR Kit
Kitartus M. tuberculosis RG PCR Kit
Sample types Human sputum, bronchoalveolar lavage (BAL), bronchial secretion, cerebral spinal fluid (CSF), stomach fluid, or peritoneal punction
Analytical sensitivity 0.23 copies/µl in the PCR
 Specificity  All members of the M. tuberculosis complex (M. tuberculosis, M. africanum, M. bovis, M. bovis BCG, M. microti, and M. pinnipedii)

Principle

artus M. tuberculosis PCR Kits are based on the amplification and simultaneous detection of a specific region of the mycobacterial genome using real-time PCR. The kits provide high levels of specificity and sensitivity.

Each artus M. tuberculosis PCR Kit provides 4 M. tuberculosis quantitation standards (see figure "Reliable quantitation of M. tuberculosis complex pathogen load"). Use of the standards enables accurate quantitation of pathogen load. In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical M. tuberculosis PCR is not reduced.

Specifications of artus M. tuberculosis RG PCR Kit
Kitartus M. tuberculosis RG PCR Kit
Sample types Human sputum, bronchoalveolar lavage (BAL), bronchial secretion, cerebral spinal fluid (CSF), stomach fluid, or peritoneal punction
Amplicon 159 bp region of the mycobacterial genome

Procedure

artus M. tuberculosis PCR Kits provide all necessary reagents optimized for reliable detection and quantitation of DNA of all members of the M. tuberculosis complex (M. tuberculosis, M. africanum, M. bovis, M. bovis BCG, M. microti, and M. pinnipedii). Simply add template DNA to the ready-to-use PCR master mix and Mg solution, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

For DNA purification, we recommend using the QIAamp DNA Mini Kit.
Applications
artus M. tuberculosis PCR Kits enable rapid and sensitive detection of DNA of members of the M. tuberculosis complex (M. tuberculosis, M. africanum, M. bovis, M. bovis BCG, M. microti, and M. pinnipedii) from sputum, bronchoalveolar lavage (BAL), bronchial secretion, cerebrospinal fluid (CSF), stomach fluid, and peritoneal punction with accurate quantification. Kits are available for use on the ABI PRISM 7000, 7700, and 7900HT SDS, on the LightCycler 1.1/1.2/1.5/2.0 Instruments, or on Rotor-Gene Q instruments.
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