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artus EBV PCR Kits RUO

For quantitative detection of Epstein-Barr virus DNA using real-time PCR
  • High reliability using the internal control
  • Sensitive detection of as few as 157 copies/ml (QS-RGQ kit)
  • Accurate quantitation using the 4 standards supplied
artus EBV PCR Kits are ready-to-use molecular detection kits for real-time PCR. The kits provide all necessary reagents optimized for EBV DNA detection and quantitation. artus EBV LC, RG, and TM PCR Kits use manual or automated sample preparation and manual assay setup techniques. The artus EBV QS-RGQ Kit is part of the QIAsymphony RGQ, the complete automated system from sample to pathogen detection, and is not for use with manual assay setup.
Cat No./ID: 4501003
artus EBV LC PCR Kit (24) RUO
For 24 reactions on the LightCycler 1.1/1.2/1.5 Instruments: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Cat No./ID: 4501103
artus EBV TM PCR Kit (24) RUO
For 24 reactions on the ABI PRISM 7000, 7700, 7900HT SDS: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Cat No./ID: 4501205
artus EBV RG PCR Kit (96) RUO
For 96 reactions on Rotor-Gene Q instruments: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Performance

To ensure sensitivity, artus EBV Kits have been optimized to detect low numbers of EBV DNA. The analytical sensitivity of the artus EBV QS-RGQ Kit is 157 copies/ml in consideration of the purification and assay setup using the QIAsymphony RGQ (see figure "Sensitive detection of EBV DNA"). 

Performance of artus EBV RG PCR Kit and artus EBV QS-RGQ Kit
Kit  artus EBV RG PCR Kitartus EBV QS-RGQ Kit
Sample types  Plasma, serum, CSF, or blood cells EDTA plasma
Analytical sensitivity  1.02 copies/μl in the PCR (Rotor-Gene 6000) 157 copies/ml in consideration of the purification

Principle

artus EBV Kits are based on the amplification and simultaneous detection of a specific region of the EBV genome using real-time PCR. The kits provide high levels of specificity and sensitivity (see figure "Sensitive detection of EBV DNA").

Each artus EBV Kit provides 4 EBV quantitation standards (see figure "Reliable quantitation of EBV load"). Use of the standards enables accurate quantitation of viral load. In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical EBV PCR is not reduced.  

Specifications of artus EBV RG PCR Kit and artus EBV QS-RGQ Kit
Kit  artus EBV RG PCR Kitartus EBV QS-RGQ Kit
Sample types  Plasma, serum, CSF, or blood cells EDTA plasma
Amplicon  97 bp region of the EBNA-1 gene 97 bp region of the EBNA-1 gene

Procedure

artus EBV PCR Kits provide all necessary reagents optimized for reliable EBV DNA detection and quantitation. Simply add template DNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

Common temperature profiles for multiple assays in one run

The temperature profile of artus EBV RG PCR Kit and the artus EBV QS-RGQ Kit corresponds to the profiles of the artus RG PCR Kits and artus QS-RGQ Kits for BK virus, CMV, HSV-1/2, and VZV. The temperature profile of the artus EBV LC PCR Kit corresponds to the profiles of the artus LC PCR Kits for HSV-1/2, VZV, and CMV. Therefore, these PCR assays can be performed and analyzed in a single run.

Complete automated system from sample to EBV detection

The QIAsymphony RGQ workflow solution for EBV detection comprises the QIAsymphony SP for sample preparation, the QIAsymphony AS for assay setup, and the artus EBV QS-RGQ Kit on the Rotor-Gene Q. The system enables reliable pathogen detection with a complete workflow (see figure "Integrated QIAsymphony RGQ system for EBV detection").

Recommendations for viral DNA purification when using the artus EBV LC, RG, or TM PCR Kits

For viral DNA purification, we recommend using the QIAamp DNA Mini Kit, QIAamp UltraSens Virus Kit, QIAamp DNA Blood Mini Kit, or EZ1 DSP Virus Kit.

Applications

artus EBV LC, RG, and TM PCR Kits enable rapid and sensitive detection of EBV DNA from serum, plasma, cerebrospinal fluid (CSF), and blood cells. Kits are available for use on the ABI PRISM 7000, 7700, and 7900HT SDS, on the LightCycler 1.1/1.2/1.5/2.0 Instruments, or on Rotor-Gene Q instruments.

The artus EBV QS-RGQ Kit is for use with EDTA plasma and is designed to be used with the QIAsymphony RGQ system, providing a complete workflow from sample to EBV DNA detection and quantitation.  

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