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artus CMV PCR Kits RUO

For quantitative detection of cytomegalovirus DNA using real-time PCR
  • High reliability using the internal control
  • Sensitive detection of as few as 42.5 copies/ml (QS-RGQ kit)
  • Accurate quantitation of viral load over a very broad linear range
artus CMV PCR Kits are ready-to-use molecular detection kits for real-time PCR. The kits provide all necessary reagents optimized for CMV DNA detection and quantitation. artus CMV LC, RG, and TM PCR Kits use automated or manual sample preparation and manual asssay setup techniques. The artus CMV QS-RGQ Kit is part of the QIAsymphony RGQ, the complete automated system from sample to pathogen detection, and is not for use with manual assay setup.
Cat No./ID: 4503003
artus CMV LC PCR Kit (24) RUO
For 24 reactions on the LightCycler 1.1/1.2/1.5/2.0 Instruments: Master, Mg Solution, 4 Quantitation Standards, Internal Control, Water (PCR grade)
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
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Reliable quantitation of CMV load.
Detection of quantitation standards (CMV QS 1–4) in fluorescence channel Cycling Green on the Rotor-Gene using the artus CMV RG PCR Kit. NTC: no template control.
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Cytomegalovirus.
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Sensitive detection of CMV DNA.
Probit analysis: CMV (QIAsymphony RGQ system). Analytical sensitivity of the artus CMV QS-RGQ Kit with the QIAsymphony RGQ system.
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Integrated QIAsymphony RGQ system for CMV detection.
Performance

To ensure sensitivity, artus CMV Kits have been optimized to detect low numbers of CMV DNA. The analytical sensitivity of the artus CMV QS-RGQ Kit is 42.5 copies/ml in consideration of the purification and assay setup using the QIAsymphony RGQ (see figure "Sensitive detection of CMV DNA"). 

Performance of artus CMV RG PCR Kit and artus CMV QS-RGQ Kit
Kit  artus CMV RG PCR Kitartus CMV QS-RGQ Kit
Sample type  EDTA plasma EDTA plasma
Analytical sensitivity  57.1 copies/ml 42.5 copies/ml
 Linear range  n.d.  79.4 to 1.00 x 108 copies/ml
n.d.: not determined.

Principle

artus CMV Kits are based on the amplification and simultaneous detection of a specific region of the CMV genome using real-time PCR. The kits provide high levels of specificity and sensitivity (see figure "Sensitive detection of CMV DNA").

Each artus CMV Kit provides 4 CMV quantitation standards (see figure "Reliable quantitation of CMV load"). Use of the standards enables accurate quantitation of viral load. In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical CMV PCR is not reduced.   

Specifications of artus CMV RG PCR Kit and artus CMV QS-RGQ Kit
Kitartus CMV RG PCR Kit and artus CMV QS-RGQ Kit
Sample type EDTA plasma
Amplicon 105 bp region of the major immediate early (MIE) gene

Procedure

artus CMV PCR Kits provide all necessary reagents optimized for reliable CMV DNA detection and quantitation. Simply add template DNA to the ready-to-use PCR master mix and Mg solution, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

Common temperature profiles for multiple assays in one run

The temperature profile of the artus CMV RG PCR Kit and the artus CMV QS-RGQ Kit corresponds to the profiles of the artus RG PCR Kits and artus QS-RGQ Kits for BK virus, EBV, HSV-1/2, and VZV. Therefore, these PCR assays can be performed and analyzed in a single run.

Complete automated system from sample to CMV detection

The QIAsymphony RGQ workflow solution for CMV detection comprises the QIAsymphony SP for sample preparation, the QIAsymphony AS for assay setup, and the artus CMV QS-RGQ Kit on the Rotor-Gene Q. The system enables reliable pathogen detection with a complete workflow (see figure "Integrated QIAsymphony RGQ system for CMV detection").

Recommendations for viral DNA purification when using the artus CMV LC, RG, or TM PCR Kits

For the artus RG and TM PCR Kits, we recommend using the QIAamp DSP Virus Kit. With the artus CMV LC PCR Kit, we recommend using either the QIAamp DSP Virus Kit or the  EZ1 DSP Virus Kit.

Applications

artus CMV LC, RG, and TM PCR Kits enable rapid and sensitive detection of CMV DNA from EDTA plasma with accurate quantification. Kits are available for use on the ABI PRISM 7000, 7700, and 7900HT SDS, on the LightCycler 1.1/1.2/1.5/2.0 Instruments, or on Rotor-Gene Q instruments.

The artus CMV QS-RGQ Kit is designed to be used with the QIAsymphony RGQ system, providing a complete workflow from sample to CMV DNA detection and quantitation.

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