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Investigator Argus X-12 QS Kit

For multiplex amplification of 12 X-chromosomal STRs, D21S11 and Amelogenin, with an innovative Quality Sensor

  • Co-amplification of 12 X-chromosomal and one autosomal alignment marker to avoid sample mix-up
  • Integrated Quality Sensor for better decision making and data interpretation
  • PCR run speed of around 80 minutes for faster results
  • Smart primer design with reduced allelic overlap for unambiguous results
  • High sensitivity and inhibitor resistance

The Investigator Argus X-12 QS Kit is a multiplex solution for human identification. The 12 X-chromosomal markers, D21S11 and Amelogenin are co-amplified. The autosomal marker, D21S11, enables alignment of the Investigator Argus X-12 QS Kit profile with a profile from any other autosomal STR kit from the expanded CODIS or ESS market, to minimize the risk of sample mix-ups. With its innovative Quality Sensor (QS), the Investigator Argus X-12 QS Kit includes a novel internal performance control. This allows you to readily determine if your PCR run was successful or if there was no DNA present in your sample. The kit utilizes QIAGEN's fast-cycling technology, enabling amplification in just ~80 minutes. Highly sensitive, with rapid and reliable results from trace or reference DNA, the Investigator Argus X-12 QS Kit is suitable for paternity or kinship testing and for gonosomal STR analysis of forensic stains.

Cat No./ID: 383223
Investigator Argus X-12 QS Kit (25)
$669.00
Add To Cart
Primer Mix, Fast Reaction Mix including Taq DNA Polymerase, Control DNA, allelic ladder Argus X-12 QS, DNA size standard 550 (BTO) and Nuclease-Free Water
Cat No./ID: 383225
Investigator Argus X-12 QS Kit (100)
$1,898.00
Add To Cart
Primer Mix, Fast Reaction Mix including Taq DNA Polymerase, Control DNA, allelic ladder Argus X-12 QS, DNA size standard 550 (BTO) and Nuclease-Free Water
The Investigator Argus X-12 QS Kit is intended for molecular biology applications in forensic, human identity and paternity testing. This product is not intended for diagnosis, prevention or treatment of a disease.
Performance
The Investigator Argus X-12 QS Kit utilizes fast-cycling technology to provide reliable results in approximately 80 minutes.
The novel Fast Reaction Mix 2.0 is based on proven QIAGEN Multiplex PCR technology that has been further optimized for direct amplification. Assay performance tests in the presence of increasing concentrations of inhibitors showed superior inhibitor resistance. This means reliable, high-quality results.
The Investigator Argus X-12 QS Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode) and the Applied Biosystems 3500 Genetic Analyzer.
Principle
The Investigator Argus X-12 QS Kit uses QIAGEN's fast-cycling PCR technology for the direct, simultaneous amplification of 12 X-chromosomal markers, D21S11 and Amelogenin. The amplification of the 12 X-chromosomal markers (see Table 1 and 2), with heightened discrimination power, meets the needs for complex kinship and paternity testing and helps fulfill the demands of complicated deficiency cases involving at least one female. The 12 X-chromosomal markers are clustered into four linkage groups (three markers per group; Table 1), and thus each set of three markers is handled as a haplotype for genotyping.

Table 1. Linkage groups
Linkage group  
1 (Xp22) DXS8378 – DS10135 – DXS10148 
2 (Xp11)  DXS7132 – DXS10074 – DXS10079 
3 (Xp26)  HPRTB – DXS10101 – DXS10103 
4 (Xp28) DXS7423 – DXS10134 – DXS10146 







The innovative Quality Sensors provides additional information on the sample quality and PCR success beyond the profile of your sample. With the Quality Sensor (QS) being amplified as an amplification control, it is possible to determine:

  • A successful PCR run
  • A failed PCR run
  • Lack of DNA in your sample

This additional information will help you analyze your data, and guides you with respect to further sample processing. It will also help you reduce unnecessary PCR re-runs, saving you time and money.
The additional autosomal marker D12S11 acts as alignment marker. Now, you have the possibility to align your X-chromosomal profile from the Investigator Argus X-12 QS Kit with a profile of any other autosomal STR kit from the expanded CODIS or ESS market, to minimize the risk of sample mix-ups.

Procedure
The 12 X-chromosomal markers, D21S11 and the gender-specific Amelogenin, are amplified simultaneously in a single PCR run and labeled with one of the dyes listed in the Table 2: "Investigator Argus X-12 QS Kit dyes and corresponding markers".
The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as Applied Biosystems GeneMapper ID-X. See the table "Technical specifications" for more information.

Table 2. Investigator Argus X-12 QS Kit dyes and corresponding markers
Dyes Markers          
6-FAM QS1 Amelogenin  DXS10103  DXS8378  DXS10101  DXS10134 
BTG DXS10074 DXS7132   DXS10135       
BTY  DXS7423  DXS10146   DXS10079       
BTR  DXSHPRTB  DXS10148  D21S11       

Table 3. Investigator Argus X-12 QS Kit technical specifications
Category Details
Volume per PCR 25 µl 
Matrix BT5
Fluorescence labels 6-FAM, BTG, BTY, BTR, BTO  
Genetic analyzers ABI PRISM 3100/3100-Avant, Applied Biosystems 3130/3130xl or
Applied Biosystems 3500/3500xL Genetic Analyzers
Software for analysis   Applied Biosystems GeneMapper ID, GeneMapper ID-X or Genotyper Software 


Applications
The Investigator Argus X-12 QS Kit enables simultaneous amplification of 12 X-chromosomal markers, and is highly suitable for:

  • Kinship and paternity testing, especially deficiency cases
  • Population genetics and anthropological studies
  • Analysis of stains with female traces on a male background

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For further information on which version of the v9 template files is suitable for your needs, please refer to the Template File Info Note that can also be found in the ''Template File'' section of the product resources.
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For further information on which version of the v9 template files is suitable for your needs, please refer to the Template File Info Note that can also be found in the ''Template File'' section of the product resources.
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