For low- to medium-throughput sample disruption for molecular analysis
- Simultaneous disruption of up to 12 samples
- Compact instrument with small footprint
- Coolable adapter to prevent biomolecule degradation
- Reproducible results with all sample types
- Compatible with all laboratory workflows
The TissueLyser LT is a small bead mill which provides fast, effective disruption of up to 12 samples at the same time. This throughput matches that of the QIAcube, which automates sample preparation using trusted QIAGEN spin-column kits. Simultaneous disruption and homogenization is achieved through high-speed shaking of samples in 2 ml microcentrifuge tubes with stainless steel or glass beads. The TissueLyser LT must be used in combination with the coolable TissueLyser LT Adapter, which holds tubes during the disruption process. Additional accessories include beads and bead dispensers.
Explore the virtual demo to learn more about the TissueLyser LT.
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TissueLyser LT
Compact bead mill, 100–240 V AC, 50–60 Hz; requires the TissueLyser LT Adapter, 12-Tube (available separately)
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85600
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TissueLyser LT Adapter, 12-Tube
Adapter for disruption of up to 12 samples in 2 ml microcentrifuge tubes on the TissueLyser LT
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69980
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TissueLyser LT は分子生物学実験用です。疾病の診断、治療または予防の目的には使用することはできません。
効果的な組織破砕|どのような解析にも使用可能なインタクトなタンパク質|動物組織からの高品質DNA|植物組織からの高品質DNA|植物組織からのインタクトなRNA|高いRIN値の高純度RNAを精製|TissueLyser LT|
TissueLyser LT あるいはTissueLyser II を用いて様々なラット組織を破砕した。[A] DNeasy Blood & Tissue Kit を用いてQIAcube でサンプル25 mg からDNA を精製した。DNA 収量は分光光度計を用いて定量した。[B] RNeasy Fibrous Tissue MiniKit(皮膚、心臓、肺)あるいはRNeasy Lipid Tissue Mini Kit(脳)を用いてQIAcube でサンプル20 mg からRNA を精製した。RNA 収量は分光光度計を用いて定量した。|Allprotect Tissue Reagent で安定化した様々なラット組織(各25 mg)を、TissueLyser LT あるいはTissueLyser II を用いて破砕した。Qproteome Mammalian Protein Prep Kit を用いてトータルタンパク質を精製し、ウエスタンブロットにより解析した。[A] Ponceau Sで染色したトランスファーしたメンブレン、[B] ウエスタンブロット。LT:TissueLyser LT; II:TissueLyser II; M: マーカー。|Allprotect Tissue Reagent で安定化した様々なラット組織(各25 mg)を、TissueLyser LT あるいはTissueLyser II を用いて破砕した。DNeasy Blood & Tissue Kit を用いてQIAcubeでDNA を精製した後、HotStarTaq Plus Master Mix Kit とPGK1 プライマーシステムでPCR を実施した。QIAxcel DNA High Resolution Kit を用いてQIAxcel で120 bp のPCR産物を解析した。LT:TissueLyser LT; II:TissueLyser II; M: マーカー。|TissueLyser LT あるいはTissueLyser II を用いて冷却済みアダプターで様々な植物組織(各100 mg)を破砕した。DNAはDNeasy Plant Mini Kit を用いてQIAcube で精製し、アガロースゲルで解析した。LT:TissueLyser LT; II:TissueLyser II; M: マーカー。|TissueLyser LT の冷却済みアダプターに様々な植物組織(各100 mg、duplicate)をセットして破砕した。RNeasy Plant Mini Kit を用いてRNA を精製し、アガロースゲルで解析した。リボゾームRNA の明確なバンドはインタクトなRNAが精製されたことを示す。|TissueLyser LT を用いてラット心臓と肺(各20 mg)を破砕した。RNeasy Fibrous Tissue Mini Kit を用いてQIAcube でRNA を精製し、Agilent 2100 Bioanalyzerで解析した。RIN 値は8.3(心臓)および9(肺)であった。||
パフォーマンス
The TissueLyser LT provides effective disruption of human, animal, and plant tissues, bacteria, and yeast, allowing reproducible yields of DNA, RNA, and protein in subsequent purification procedures (see figure "Effective tissue disruption"). Even difficult-to-lyse tissues such as heart and brain can be processed by the TissueLyser LT. DNA, RNA, and protein remain intact after disruption (see figures "Pure RNA with high RIN values", "Intact protein suitable for all types of analysis", "High-quality DNA from animal tissues", "High-quality DNA from plant tissues", and "Intact RNA from plant tissues"), enabling reliable analysis in downstream applications.
Disruption with the TissueLyser LT is comparable to that achieved with the well-established TissueLyser II (see figures "Effective tissue disruption", "Intact protein suitable for all types of analysis", "High-quality DNA from animal tissues", and "High-quality DNA from plant tissues").
原理
Genotyping, gene expression, and proteomics applications demand effective disruption of biological samples to ensure high yields of DNA, RNA, and protein. The TissueLyser LT system delivers thorough and rapid disruption of samples to fully release biomolecules, and also simultaneously homogenizes samples to facilitate subsequent purification procedures using QIAGEN kits (see table "QIAGEN purification kits compatible with QIAGEN disruption systems").
The TissueLyser LT is an integral part of QIAGEN's complete solution for sample management — from sample collection to purification and analysis of DNA, RNA, and protein. The TissueLyser LT is fully compatible with QIAGEN manual sample preparation kits (see table "QIAGEN purification kits compatible with QIAGEN disruption systems"), and also complements QIAGEN's range of automated solutions for medium-throughput sample preparation and analysis (see table "QIAGEN medium-throughput automation"). These include the QIAcube, which automates sample purification using QIAGEN spin-column kits, and the QIAxcel, which automates multicapillary gel electrophoresis of DNA and RNA.
| RNA |
Easy-to-lyse tissue |
RNeasy Kits |
| RNeasy Plus Kits |
| RNeasy Protect Kits |
| RNA |
Fiber-rich tissue |
RNeasy Fibrous Tissue Kits |
| RNA |
All types of tissue |
RNeasy Lipid Tissue Kits |
| RNeasy Universal Tissue Kits |
| RNA |
Plant tissue |
RNeasy Plant Mini Kit |
| RNA |
Yeast |
RNeasy Kits |
| RNA |
Bacteria |
RNeasy Protect Bacteria Kits |
| microRNA |
All types of tissue |
miRNeasy Kits |
| DNA |
Human tissue |
QIAamp DNA Kits |
| DNA |
Animal tissue |
DNeasy Blood & Tissue Kits |
| Protein |
Tissue |
Qproteome Mammalian Protein Prep Kit |
| Phosphoprotein |
Tissue |
PhosphoProtein Purification Kit |
| Glycoprotein |
Tissue |
Qproteome Glycoprotein Kits |
| DNA and RNA |
Tissue |
AllPrep DNA/RNA Kits |
| DNA, RNA, and protein |
Tissue |
AllPrep DNA/RNA/Protein Mini Kit |
| QIAcube |
Purification of DNA, RNA, and protein |
Up to 12 samples per run |
| EZ1 Advanced |
Purification of DNA and RNA from human samples |
Up to 6 samples per run |
| EZ1 Advanced XL |
Purification of DNA and RNA from human samples |
Up to 14 samples per run |
| QIAxcel |
Electophoretic analysis of DNA fragments and RNA |
Up to 96 samples per run |
| QIAgility |
Reaction setup |
Up to 384 samples per run |
| Rotor-Gene Q |
Real-time PCR and high-resolution melting (HRM) analyses |
Up to 100 samples per run |
| PyroMark Q24 |
Methylation and mutation analyses |
Up to 24 samples per run |
操作手順
The TissueLyser LT works in combination with the TissueLyser LT Adapter. Up to twelve 2 ml tubes, each containing a sample and a bead, are loaded into the adapter, which is securely fastened to the piston of the TissueLyser LT. The piston moves up and down rapidly, leading to simultaneous disruption and homogenization of the samples due to the beating and grinding action of the beads.
The TissueLyser LT has a small footprint of 15 cm x 27 cm, allowing installation in any laboratory. As each sample is safely sealed within its own tube, the TissueLyser LT is able to disrupt multiple samples without any risk of cross-contamination. If disrupting fresh or frozen samples, the TissueLyser LT Adapter can be precooled on dry ice to prevent degradation of nucleic acids and proteins (see figures "High-quality DNA from plant tissues" and "Intact RNA from plant tissues"). Tissues stored in Allprotect Tissue Reagent (to stabilize DNA, RNA, and protein) or in RNAprotect Tissue Reagent (to stabilize RNA) require no precooling of the adapter.
アプリケーション
The ability to process up to 12 samples per run makes the TissueLyser LT the ideal front-end solution to access biological information for genomics, transcriptomics, and proteomics applications. The TissueLyser LT enables fast and uniform disruption of animal and human tissues, plant tissues, bacteria, and yeast in the TissueLyser LT Adapter, which holds up to twelve 2 ml sample tubes. Sample purification can be performed manually, or can be automated using the QIAcube, QIAsymphony SP, QIAxtractor, EZ1 Advanced, EZ1 Advanced XL, or BioRobot, or BioSprint instruments.
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Feature
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Specifications
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Dimensions
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Weight: Approx. 7 kg, Width: 150 mm, Depth: 270 mm, Height: 280 mm, The power supply of the TissueLyser LT is compatible with voltages of 100–240 V
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Disruption principle
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High-speed shaking of samples in 2 ml microcentrifuge tubes with stainless steel or glass beads
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Features
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Simultaneous disruption of up to 12 samples. Compact instrument with small footprint. Coolable adapter to prevent biomolecule degradation. Reproducible results with all sample types. Compatible with all laboratory workflows.
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Kits compatible with instrument
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All kits for purification of RNA/DNA/protein
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Protocol/main application on this intrument
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Sample preparation/sample disruption
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Technology
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Bead Mill
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Typical run time
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40 sec - 5 min, depending on protocol
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FAQ ID -2545
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FAQ ID -2546
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FAQ ID -2551
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FAQ ID -2552
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FAQ ID -2555
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FAQ ID -374
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FAQ ID -2548
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For disruption of up to 12 samples on the TissueLyser LT
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詳細を表示
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For disruption of up to 12 biological samples
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詳細を表示
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詳細を表示
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詳細を表示
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最高12生体サンプルの破砕
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詳細を表示
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画像
効果的な組織破砕
TissueLyser LT あるいはTissueLyser II を用いて様々なラット組織を破砕した。[A] DNeasy Blood & Tissue Kit を用いてQIAcube でサンプル25 mg からDNA を精製した。DNA 収量は分光光度計を用いて定量した。[B] RNeasy Fibrous Tissue MiniKit(皮膚、心臓、肺)あるいはRNeasy Lipid Tissue Mini Kit(脳)を用いてQIAcube でサンプル20 mg からRNA を精製した。RNA 収量は分光光度計を用いて定量した。
どのような解析にも使用可能なインタクトなタンパク質
Allprotect Tissue Reagent で安定化した様々なラット組織(各25 mg)を、TissueLyser LT あるいはTissueLyser II を用いて破砕した。Qproteome Mammalian Protein Prep Kit を用いてトータルタンパク質を精製し、ウエスタンブロットにより解析した。[A] Ponceau Sで染色したトランスファーしたメンブレン、[B] ウエスタンブロット。LT:TissueLyser LT; II:TissueLyser II; M: マーカー。
動物組織からの高品質DNA
Allprotect Tissue Reagent で安定化した様々なラット組織(各25 mg)を、TissueLyser LT あるいはTissueLyser II を用いて破砕した。DNeasy Blood & Tissue Kit を用いてQIAcubeでDNA を精製した後、HotStarTaq Plus Master Mix Kit とPGK1 プライマーシステムでPCR を実施した。QIAxcel DNA High Resolution Kit を用いてQIAxcel で120 bp のPCR産物を解析した。LT:TissueLyser LT; II:TissueLyser II; M: マーカー。
植物組織からの高品質DNA
TissueLyser LT あるいはTissueLyser II を用いて冷却済みアダプターで様々な植物組織(各100 mg)を破砕した。DNAはDNeasy Plant Mini Kit を用いてQIAcube で精製し、アガロースゲルで解析した。LT:TissueLyser LT; II:TissueLyser II; M: マーカー。
植物組織からのインタクトなRNA
TissueLyser LT の冷却済みアダプターに様々な植物組織(各100 mg、duplicate)をセットして破砕した。RNeasy Plant Mini Kit を用いてRNA を精製し、アガロースゲルで解析した。リボゾームRNA の明確なバンドはインタクトなRNAが精製されたことを示す。
高いRIN値の高純度RNAを精製
TissueLyser LT を用いてラット心臓と肺(各20 mg)を破砕した。RNeasy Fibrous Tissue Mini Kit を用いてQIAcube でRNA を精製し、Agilent 2100 Bioanalyzerで解析した。RIN 値は8.3(心臓)および9(肺)であった。
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