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EZ1 DSP Virus Kit

For automated, simultaneous purification of viral nucleic acids and bacterial DNA from 1–6 or 1–14 human biological specimens using the EZ1 Advanced or the EZ1 Advanced XL
  • High analytical sensitivity even with low viral titers
  • Rapid and flexible purification of 1–6 or 1–14 samples per run
  • Easy protocol setup with credit-card ease of use
  • Standardized processing ensures reproducible results
 The EZ1 DSP Virus Kit contains all required reagents and labware for automated purification of viral nucleic acids and bacterial DNA from human serum, plasma, cerebrospinal fluid (CSF), urine, whole blood, stool, transport media, respiratory samples, and dried swabs using magnetic-particle technology. Reagents are supplied in prefilled reagent cartridges, which ensures speed and convenience in loading the EZ1 Advanced or the EZ1 Advanced XL. The EZ1 DSP Virus Kit provides exact performance specifications, assuring highly reliable viral nucleic acid purification.

The EZ1 DSP Virus Kit is intended for in vitro diagnostic use.


Principle
Automated sample preparation enables efficient, standardized purification of nucleic acids. The EZ1 Advanced and the EZ1 Advanced XL enable purification of nucleic acids using proven magnetic-particle technology for use in routine in vitro diagnostic applications. Reagent production is subjected to stringent quality control assuring reliable and robust performance.
An easy-to-use instrument, prefilled and sealed reagent cartridges, and simple protocol selection and worktable setup help to prevent handling errors and allow the system to be operated by anyone — from the novice to the expert. Manual handling of potentially infectious samples is minimized, ensuring safety to the user and reliable processing of samples. Purified viral DNA and RNA are ready to use in sensitive downstream diagnostic assays based on enzymatic amplification, such as PCR and reverse transcriptase PCR (RT-PCR) for viral load monitoring.
Procedure
The EZ1 Advanced or the EZ1 Advanced XL in combination with the proven EZ1 DSP Virus Kit enables purification of viral nucleic acids and bacterial DNA from 1–6 (EZ1 Advanced) or 1–14 (EZ1 Advanced XL) samples of human serum, plasma, CSF, urine, whole blood, stool, transport media, respiratory samples, and dried swabs. All processing steps are performed by the instrument, from piercing the reagent cartridge to elution of highly pure nucleic acids. The innovative design of the instrument means that the pipet tips function as separation chambers, increasing the efficiency of magnetic purification and eliminating the need for centrifugation steps. The streamlined procedure provides robust walkaway purification of nucleic acids with minimal hands-on time.
Applications
In combination with the EZ1 Advanced DSP Virus Card and the EZ1 Advanced or the the EZ1 Advanced XL DSP Virus Card and the EZ1 Advanced XL, the EZ1 DSP Virus Kit provides a fully automated procedure for simultaneous purification of viral nucleic acids and bacterial DNA from human serum, plasma, CSF, urine, whole blood, stool, transport media, respiratory samples, and dried swabs. Viral nucleic acids purified using the EZ1 DSP Virus Kit are ready to use in downstream diagnostic assays based on enzymatic amplification, such as PCR and RT-PCR.
Features
Specifications
Applications PCR, real-time RT-PCR, genotyping
CE/FDA/IVD compatible CE/IVD
Elution volume 75–50 µl
For automated processing BioRobot EZ1
Format Sample tube
Main sample type Serum, plasma
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Viral DNA, viral RNA
Sample amount 100–400 µl
Technology Magnetic particles
Time per run or per prep 40 minutes
Yield Varies
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