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EZ1 DNA Buffy Coat Card

For easy setup of protocols using the EZ1 DNA Blood 350 µl Kit on the BioRobot EZ1 workstation
  • Easy protocol setup with credit card ease of use
  • Standardized processing ensures reproducible results
  • Optimized protocols help to ensure reliable purification
  • Automated purification of genomic DNA

The EZ1 DNA Buffy Coat Card is preprogrammed with protocols for purification of DNA from buffy coat using the EZ1 DNA Blood 350 µl Kit on the BioRobot EZ1.

Cat No./ID: 9015587
EZ1 DNA Buffy Coat Card
$805.00
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Pre-programmed card for BioRobot EZ1 DNA Buffy Coat Protocol

EZ1 Cards are used in combination with EZ1 Kits on the BioRobot EZ1. Please refer to the specific EZ1 Kit handbook and the BioRobot EZ1 User Manual for information about product use.



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Consistent PCR results.
Amplification of a 900 bp single-copy gene (MECL-1) using template DNA purified from 8 different buffy coat samples with low to medium WBC concentration. Total DNA elution volume was 200 µl and 5 µl DNA was used as template in 50 µl PCR. A 5 µl (10%) aliquot of each PCR was visualized using a 1.5% agarose gel. M: 100 bp DNA ladder (100 ng); +: positive control; -: negative control.
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Representative yields of high-purity DNA from buffy coat samples.
Each data point represents the average yield from 3 replicates.
Performance

The yield and quality purified DNA are suited for use in many applications (see figures "Representative yields of high-purity DNA from buffy coat samples") and "Consistent PCR results").

Principle
EZ1 Cards specify a choice of purification protocols without any manual data entry. Further EZ1 Cards may be purchased at any time  increasing the application range of the BioRobot EZ1.
Procedure

Simply insert the EZ1 Card with the desired protocol into the EZ1 Card slot and start the instrument.

Applications

The high-quality DNA obtained using the BioRobot EZ1 with the EZ1 DNA Blood 350 µl Kit and the EZ1 DNA Buffy Coat Card is suited for use in many applications, such as:

Population genetics and pharmacogenomic research
PCR and real-time PCR
Genotyping
Sequencing
Oncology research
SNP, STR, VNTR, RAPD, NASBA, and AFLP technologies
Features
Specifications
Applications PCR, real-time RT-PCR, genotyping
For automated processing BioRobot EZ1
Processing Automated
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