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EZ1 DNA Blood Card

For easy setup of protocols using EZ1 DNA Blood Kits on the BioRobot EZ1

  • Easy protocol setup with credit-card ease of use
  • Standardized processing ensures reproducible results
  • Optimized protocols help to ensure reliable purification
  • Automated purification of genomic DNA

The EZ1 DNA Blood Card is preprogrammed with protocols for purification of DNA from blood and blood-derived samples using the EZ1 DNA Blood 200 µl Kit or EZ1 DNA Blood 350 µl Kit on the BioRobot EZ1.

Cat No./ID: 9015585
EZ1 DNA Blood Card
$805.00
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Pre-programmed card for BioRobot EZ1 DNA Blood 200 µl and 350 µl Protocols

EZ1 Cards are used in combination with EZ1 Kits on the BioRobot EZ1. Please refer to the specific EZ1 Kit handbook and the BioRobot EZ1 User Manual for information about product use.



0
Reproducible performance in sensitive PCR analysis.
Amplification of a single-copy gene (MECL-1) using template DNA purified from whole blood. Upper and lower lanes are from PCR using template DNA from 200 µl and 350 µl blood samples, respectively. Lanes 1–6 and 7–12 are from the first and last of 8 processing runs on the BioRobot EZ1, respectively. Blood from the same donor is used in all isolations. M: 100 bp DNA ladder (100 ng); +: positive control; –: negative control. 2 µl (1%) of each PCR was loaded on the agarose gel.
1
Sensitive PCR using DNA purified from preserved blood.
PCR of a 900 bp fragment of the MECL-1 single-copy gene using serial dilutions of template DNA purified from 200 µl ACD-, heparin-, and EDTA-preserved whole blood. Purified DNA (15-0.25 µl) was used in each 50 µl PCR as indicated. M: 100 bp DNA ladder; C: negative control.
Performance

The purified nucleic acids are suited for use in many applications (see figures "Reproducible performance in sensitive PCR analysis" and "Sensitive PCR using DNA purified from preserved blood").

Principle
EZ1 Cards specify a choice of purification protocols without any manual data entry. Further EZ1 Cards may be purchased at any time  increasing the application range of the BioRobot EZ1.
Procedure

Simply insert the EZ1 Card with the desired protocol into the EZ1 Card slot and start the instrument.

Applications

The high-quality DNA obtained using the BioRobot EZ1 with the 
EZ1 DNA Blood 200 µl Kit or the EZ1 DNA Blood 350 µl Kit with the EZ1 DNA Blood Card is suited for use in many applications, such as:

Population genetics and pharmacogenomic research
PCR and real-time PCR
Genotyping
Sequencing
Oncology research
SNP, STR, VNTR, RAPD, NASBA, and AFLP technologies

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Kit Handbooks (1)
EZ1 DNA Blood 200 μl Kit
EZ1 DNA Blood 350 μl Kit
For automated purification of DNA from blood and buffy coat using EZ1 instruments
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Safety Data Sheets (1)
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