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App. Package, M48, Inf. Dis. (CD)

For easy setup of protocols for infectious disease research
  • Easy protocol setup with all protocols on one CD-ROM
  • Optimized protocols help to ensure reliable purification
  • Range of protocols increases processing flexibility

The App. Package, M48, Inf. Dis. is a CD-ROM containing protocols for bacterial DNA purification from culture and primary samples and viral nucleic acid purification from plasma, serum, and CSF samples using MagAttract M48 Kits on the BioRobot M48 workstation. These protocols provide operating commands for the work-station and comprehensive, on-screen instructions for easy setup.

Cat No./ID: 9016145
App. Package, M48, Infectious Disease
Software protocol package for infectious disease applications, v. 3.0, on the BioRobot M48 workstation
The App. Package, M48, Inf. Dis. (CD) is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Highly reproducible purification.
A negative plasma pool was spiked on each of 3 days with a typical DNA or RNA virus. Viral nucleic acids were purified from 24 aliquots (400 µl each) of each pool using the MagAttract Virus Mini M48 Kit, with elution in a volume of 125 µl. [A] Pools were spiked with 105 copies/ml of a typical DNA virus. Purified viral DNA (10 µl of each eluate) was analyzed by real-time PCR using the QuantiTect SYBR® Green PCR Kit, and CT values are shown for 24 samples on each day. [B] Pools were spiked with 104 copies/ml of a typical RNA virus. Purified viral RNA (10 µl of each eluate) was analyzed by real-time RT-PCR using the QuantiTect Probe RT-PCR Kit, and CT values are shown for 24 samples on each day.
Performance
The App. Package, M48, Inf. Dis. with MagAttract M48 Kits can be used to purify nucleic acids from a broad range of DNA and RNA viruses or DNA from a broad range of bacteria for life science applications (see figure "Highly reproducible purification").
Principle

The BioRobot M48 and MagAttract M48 Kits, with protocols on the App. Package, M48, Inf. Dis., reproducibly automate purification of bacterial DNA and viral nucleic acids. Purification of 6–48 samples per run is efficient and purified nucleic acids perform well in downstream analyses.

The BioRobot M48 system provides fully automated purification of nucleic acids using silica-coated magnetic particles, eliminating the need for tedious centrifugation steps. The easy-to-use workstation allows purification of 6–48 samples in a single run. Between processing runs, the worktable can be decontaminated using an integrated UV lamp to reduce the chances of environmental contamination.

Procedure
Protocols on the App. Package, M48, Inf. Dis., V3.2
Sample type Amount KitNucleic acid
Bacterial cultures 200 µl MagAttract DNA Mini M48 Kit Bacterial DNA
Primary sample material 200 µl MagAttract DNA Mini M48 Kit Bacterial DNA
Serum or plasma 300 µl MagAttract Viral RNA M48 Kit Viral RNA
Serum or plasma 50 μl, 100 μl, 200 μl, or 400 µl MagAttract Virus Mini M48 Kit Viral DNA and RNA
Applications

Using the App. Package, M48, Inf. Dis. with the MagAttract Virus Mini M48 Kit provides a fully automated procedure for simultaneous purification of viral DNA and RNA for highly sensitive detection in downstream assays, and using the App. Package, M48, Inf. Dis. with the MagAttract DNA Mini M48 Kit provides a fully automated procedure for purification of bacterial DNA. The kits can be used to purify nucleic acids from a broad range of DNA and RNA viruses or DNA from a broad range of bacteria for life science applications.

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