text.skipToContent text.skipToNavigation

App. Package, M48, Genotyping

For easy setup of protocols for genotyping applications
  • Easy protocol setup with all protocols on one CD-ROM
  • Optimized protocols help to ensure reliable purification
  • Range of protocols increases processing flexibility

The App. Package, M48, Genotyping is a CD-ROM containing protocols for DNA purification from 100–700 μl blood or 25–300 μl buffy coat samples using MagAttract DNA Blood M48 Kits on the BioRobot M48 workstation. These protocols provide operating commands for the workstation and allow easy setup through comprehensive, on-screen instructions.

Cat No./ID: 9016146
App. Package, M48, Genotyping
Software protocol package for genotyping applications on the BioRobot M48 workstation
The App. Package, M48, Genotyping is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
0
Range of yields and concentrations from blood samples.
[A] Average DNA yields from 350 µl blood samples using 50–400 µl elution volumes. Six replicates were purified and analyzed for each elution volume. [B] Average DNA concentration from 350 µl blood samples, using 100–400 µl elution volumes.
Performance
DNA yields depend on the sample type, number of nucleated cells in the sample, and the protocol used for isolation of DNA (see figure "Range of yields and concentrations from blood samples").
Principle

The BioRobot M48 and the MagAttract DNA Mini M48 Kit, with protocols on the App. Package, M48, Genotyping, reproducibly automate purification of total (genomic and mitochondrial) DNA from human whole blood and blood products. Purification of 6–48 samples per run is efficient and purified DNA performs well in downstream analyses.

The BioRobot M48 system provides fully automated purification of DNA using silica-coated magnetic particles, eliminating the need for tedious centrifugation steps. The easy-to-use workstation allows purification of 6–48 samples in a single run. Between processing runs, the worktable can be decontaminated using an integrated UV lamp to reduce the chances of environmental contamination.

Procedure
Protocols on the App. Package, M48, Genotyping, V1.0
Sample typeAmount Kit Nucleic acid
Blood 100, 150, or 200 µl MagAttract DNA Blood Mini M48 Kit DNA
Blood 250, 300, or 350 µl MagAttract DNA Blood Mini M48 Kit DNA
Blood 500, 600, or 700 µl MagAttract DNA Blood Mini M48 Kit DNA
Buffy coat 25, 50, or 75 µl MagAttract DNA Blood Mini M48 Kit DNA
Buffy coat 75, 100, or 150 µl MagAttract DNA Blood Mini M48 Kit DNA
Buffy coat 200 or 300 µl MagAttract DNA Blood Mini M48 Kit DNA
Applications

The high-quality DNA obtained using MagAttract DNA Blood M48 Kits with protocols in the App. Package, M48, Genotyping is suitable for use in many downstream applications, such as:

Genotyping by STR, VNTR, RAPD, NASBA, and AFLP technologies
Population genetics
Pharmacogenomic research
fragment fix placeholder