PyroMark Q24 Advanced Accessories and Reagents

For optimal sample preparation and Pyrosequencing analysis using the PyroMark Q24 Advanced

Improved Pyrosequencing chemistry for long sequence runs
Reliable quantification of genetic or epigenetic DNA modifications
Solutions for quick and easy sample handling for successful results
Dedicated products for each platform and each step in the workflow
High-quality optimized reagents enable reliable Pyrosequencing results

The PyroMark Q24 Advanced uses proven Pyrosequencing technology for real-time, sequence-based detection and quantification for genetic analysis and epigenetic methylation studies. PyroMark Q24 Advanced Accessories and Reagents are high-quality reagents and products that simplify and streamline the Pyrosequencing workflow and enable successful results. PyroMark Q24 Advanced Accessories and Reagents are designed specifically for use with the PyroMark Q24 Advanced system.

Informações sobre pedidos
Recursos do Produto

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Produto	Num Cat.	Preço de lista:
PyroMark CpG Assay (200) PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)	Varies	Details
PyroMark CpG Assay 96 well (200) PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; plate format); minimum order of 24 assays per plate	Varies	Details
PyroMark CpG Assay 96 well (50) PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (50 reactions; plate format); minimum order of 24 assays per plate	Varies	Details
PyroMark Custom Assay (200) User-designed PCR and sequencing primers for Pyrosequencing analysis (200 reactions; tube format)	Varies	Details
PyroMark Denaturation Sol. (500 ml) For denaturation of double-stranded PCR product into single-stranded DNA template	979007	R$417,00	Adicionar ao carrinho
PyroMark OneStep RT-PCR Kit (200) For 200 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water	978803	Log in
PyroMark OneStep RT-PCR Kit (50) For 50 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water	978801	Log in
PyroMark PCR Kit (200) For 200 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl₂ and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl₂, and RNase-Free Water.	978703	R$1.993,00	Adicionar ao carrinho
PyroMark PCR Kit (800) For 800 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl₂ and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl₂, and RNase-Free Water	978705	Log in
PyroMark Q24 Advanced Reagents (4 x 24) Enzyme mix, substrate mix, nucleotides, and buffers for Pyrosequencing assays; intended for use with PyroMark Q24 Advanced	970902	R$2.478,00	Adicionar ao carrinho
PyroMark Q24 Advanced CpG Reagents (4 x 24) Enzyme mix, substrate mix, nucleotides, and buffers for CpG and long read Pyrosequencing assays; intended for use with PyroMark Q24 Advanced	970922	Log in
PyroMark Q24 Cartridge (3) Reusable dispensing tool for nucleotide and reagent delivery, for use with PyroMark Q24 and PyroMark Q24 Advanced	979202	R$3.502,00	Adicionar ao carrinho
PyroMark Q24 Plate (100) 24-well plate for samples, for use with PyroMark Q24 and PyroMark Q24 Advanced; 100 plates in each package	979201	R$1.702,00	Adicionar ao carrinho
PyroMark Q24 Vacuum Prep Troughs (12) Reusable plastic troughs for use with all PyroMark Q24 Vacuum Workstations	979206	R$346,00	Adicionar ao carrinho
PyroMark Q24 Vacuum Workstation (100 V) Vacuum Workstation for preparing 24 samples in parallel from PCR product to single-stranded template	9001519	Cotação
PyroMark Q24 Vacuum Workstation (110 V) Vacuum Workstation for preparing 24 samples in parallel from PCR product to single-stranded template	9001516	Cotação
PyroMark Q24 Vacuum Workstation (220 V) Vacuum Workstation for preparing 24 samples in parallel from PCR product to single-stranded template	9001518	Cotação
PyroMark Vacuum Prep Filter Probe (100) Reusable filter probes for PyroMark Vacuum Workstation Q96 and Q24	979010	R$2.479,00	Adicionar ao carrinho
PyroMark Wash Buffer (conc., 200 ml) For use with the PyroMark Q24 Vacuum Workstation and PyroMark Q96 Vacuum Workstation to wash and neutralize the immobilized DNA	979008	R$416,00	Adicionar ao carrinho

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PyroMark Q24 Advanced Accessories and Reagents are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Brochures & Guides

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	Pyrosequencing – the synergy of sequencing and quantification	Mostrar detalhes
	Product Profile - PyroMark Q24 Advanced	Mostrar detalhes

FAQs

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	What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set? FAQ ID -2007	Visualizar
	How do I set up a PyroMark CpG Assay? FAQ ID -2814	Visualizar
	How are the PyroMark CpG Assays shipped and stored? FAQ ID -2816	Visualizar
	How are the PyroMark CpG Assays reconstituted? FAQ ID -2817	Visualizar
	Does QIAGEN offer a design of Custom PyroMark CpG Assays? FAQ ID -2818	Visualizar
	What are the features of PyroMark CpG Assays e.g. in terms of design, validation? FAQ ID -2821	Visualizar
	Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays? FAQ ID -2822	Visualizar
	Will the primer sequence for the PyroMark CpG Assay be provided? FAQ ID -2823	Visualizar
	In which format can PyroMark CpG Assays be ordered? FAQ ID -2824	Visualizar
	What is the recommended amplicon size for CpG assays? FAQ ID -2825	Visualizar
	What concentration should be used for the sequencing primer in pyrosequencing? FAQ ID -2826	Visualizar
	Can I order the nucleotides from PyroMark Gold Reagents separately? FAQ ID -2827	Visualizar
	Which purity grade is recommended for pyrosequencing primers? FAQ ID -2832	Visualizar
	Is the CpG software included in the PyroMark instruments to study methylation status? FAQ ID -2842	Visualizar
	How many times can vacuum troughs be re-used with the PyroMark Vacuum Preparation Stations? FAQ ID -2848	Visualizar
	Where can I order the Streptavidin Sepharose beads for pyrosequencing? FAQ ID -2850	Visualizar
	How many nucleotides of a homopolymer can be resolved in pyrosequencing? FAQ ID -2871	Visualizar
	Can unused wells in a pyrosequencing plate be used in the next run? FAQ ID -2872	Visualizar
	What is the reason for signals ceasing in the middle of a pyrosequencing run? FAQ ID -2875	Visualizar
	How do I reduce background peaks in the pyrosequencing pyrogram? FAQ ID -2877	Visualizar
	How do I prevent a drifting baseline in my pyrosequencing pyrogram? FAQ ID -2878	Visualizar
	What is the reason for a high substrate peak in the pyrosequencing pyrogram? FAQ ID -2879	Visualizar
	What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram? FAQ ID -2881	Visualizar
	What is included in a PyroMark Custom Assay? FAQ ID -2815	Visualizar
	Which end of the PCR primer for pyrosequencing should be biotinylated? FAQ ID -2839	Visualizar
	Will dUTP in a PCR reaction affect pyrosequencing? FAQ ID -2843	Visualizar
	Does the Pyromark Assay Design or application software give any cycling conditions for individual assay primers or a PCR setup pipetting scheme? FAQ ID -2862	Visualizar
	Is it possible to use PyroMark Q24 Advanced reagents on PyroMark Q24 system? FAQ ID -3160	Visualizar
	What are the differences between new PyroMark Q24 Advanced reagents chemistry compared to PyroMark Q24 Gold reagents? FAQ ID -3165	Visualizar
	How accurate and reliable is PyroMark Q24 in mutation analysis? FAQ ID -2094	Visualizar
	What kind of shaker should be used for the pyrosequencing binding step? FAQ ID -2837	Visualizar
	Which heating block is recommended for the pyrosequencing annealing step? FAQ ID -2838	Visualizar
	Can PyroMark Gold reagents be vortexed? FAQ ID -2844	Visualizar
	What is the concentration of PyroMark Control Oligo? FAQ ID -2846	Visualizar
	What should be the single peak height for the PyroMark Control Oligo on the different PyroMark instruments? FAQ ID -2852	Visualizar
	What is the use of the PyroMark Q24 Validation Oligo? FAQ ID -2855	Visualizar
	How many times can the cartridges for Pyromark Q24 or PyroMark Q96 ID instruments are reused? FAQ ID -2863	Visualizar

Additional Resources

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	(EN) - Offline Ordering Instructions for PyroMark CpG Assays	Mostrar detalhes
	(EN) - Offline Ordering Instructions for PyroMark Custom Assays	Mostrar detalhes

Scientific Posters

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	(EN) - PyroMark CpG Assays: A new tool for genome-wide methylation profiling by Pyrosequencing	Mostrar detalhes
	Advanced DNA methylation analysis for verification of Infinium HumanMethylation450 BeadChip data Scientific poster	Mostrar detalhes
	(EN) - Pyrosequencing and its applications	Mostrar detalhes

User-Developed Protocols

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	Pyrosequencing® method for the forensic identification of tissue source using QIAGEN’s PyroMark® Q24 Advanced system - (EN)	Mostrar detalhes

White Papers

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	(EN) - Techniques to overcome bottlenecks in epigenetics research	Mostrar detalhes

Safety Data Sheets

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	Safety Data Sheets Download Safety Data Sheets for QIAGEN product components.	Visualizar

Kit Handbooks

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	(EN) - PyroMark OneStep RT-PCR Handbook	Mostrar detalhes
	(EN) - PyroMark PCR Handbook	Mostrar detalhes
	PyroMark Q24 Advanced & Advanced CpG Reagents Handbook	Mostrar detalhes
	(EN) - PyroMark Q24 Cartridge Product Sheet	Mostrar detalhes

Application Notes

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	Tissue source attribution using the PyroMark Q48 Autoprep System: Sperm identification in forensic casework	Mostrar detalhes
	Pyrosequencing – tissue source forensic identification using PyroMark Q24 Advanced A Pyrosequencing method for the forensic identification of tissue source using QIAGEN’s PyroMark Q24 Advanced system.	Mostrar detalhes

Quick-Start Protocols

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	(EN) - PyroMark PCR Kit Quick-Start Protocol	Mostrar detalhes
	PyroMark Q24 Advanced Reagents Quick-Start Protocol, Part 1 (EN)	Mostrar detalhes
	PyroMark Q24 Advanced Reagents Quick-Start Protocol, Part 2 (EN)	Mostrar detalhes
	PyroMark Q24 Advanced CpG Reagents Quick-Start Protocol, Part 1 (EN)	Mostrar detalhes
	PyroMark Q24 Advanced CpG Reagents Quick-Start Protocol, Part 2 (EN)	Mostrar detalhes
	(EN) - PyroMark Q24 Vacuum Workstation Quick-Start Guide	Mostrar detalhes

Download Files

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	(EN) - PyroMark Q24 Advanced Method 0003 Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0003	Mostrar detalhes
	(EN) - PyroMark Q24 Advanced Method 0004 Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0004	Mostrar detalhes
	(EN) - PyroMark Q24 Advanced Method 0005 Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0005	Mostrar detalhes
	(EN) - PyroMark Q24 Advanced Method 0006 Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0006	Mostrar detalhes
	(EN) - PyroMark Q24 Advanced Method 0007 Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0007	Mostrar detalhes
	(EN) - PyroMark Q24 Advanced Method 0008 Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0008	Mostrar detalhes
	(EN) - PyroMark Q24 Advanced Method 0009 Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0009	Mostrar detalhes
	(EN) - PyroMark Q24 Advanced Method 0010 Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0010	Mostrar detalhes
	(EN) - PyroMark Q24 Advanced Method 0011 Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0011	Mostrar detalhes
	(EN) - PyroMark Q24 Advanced Method 0012 Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0012	Mostrar detalhes
	(EN) - PyroMark Q24 Advanced Method 0013 Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0013	Mostrar detalhes

Fichas de Segurança

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	MSDS PyroMark CpG Assay (200)
	MSDS PyroMark CpG Assay 96 well (200)
	MSDS PyroMark CpG Assay 96 well (50)
	MSDS PyroMark Custom Assay (200)
	MSDS PyroMark Denaturation Sol. (500 ml)
	MSDS PyroMark OneStep RT-PCR Kit (200)
	MSDS PyroMark OneStep RT-PCR Kit (50)
	MSDS PyroMark PCR Kit (200)
	MSDS PyroMark PCR Kit (800)
	MSDS PyroMark Q24 Advanced Reagents (4 x 24)
	MSDS PyroMark Q24 Advanced CpG Reagents (4 x 24)
	MSDS PyroMark Q24 Cartridge (3)
	MSDS PyroMark Vacuum Prep Filter Probe (100)
	MSDS PyroMark Wash Buffer (conc., 200 ml)

Imagens