Qproteome Total Glycoprotein Kit

For isolation of glycosylated proteins
  • Highly specific separation of glycoproteins
  • Profiling of glycoproteins
  • Range of lectin columns for comprehensive glycoprotein characterization

The kit contains lectin-resin filled spin columns, buffers, and reagents for the isolation of glycoproteins from cell lysate or serum samples.

Spin Column Adapter Rings are required for automation of this kit on the QIAcube.

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Product Cat. no. List price:
Qproteome Total Glycoprotein Kit
For 6 total glycoprotein preps: Buffers, Lectin Spin Columns (6), Detergent Solution, Protease Inhibitor Solution, Collection Tubes (6 x 2 ml)
 37541
$526.00
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The Qproteome Total Glycoprotein Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Glycoprotein fractionation procedure.|Highly specific glycoprotein fractionation.|
|Glycoproteins were fractionated from serum using the different lectin spin columns in glycoprotein fractionation kits and analyzed by SDS-PAGE followed by silver staining. [A] Elution steps 1–3 and 4–6 from Total Lectin Spin Columns in the Total Glycoprotein Kit. [B] Eluted glycoproteins from ConA, GNA, and LCH Spin Columns in the Mannose Glycoprotein Kit. [C] Eluted glycoproteins from WGA, SNA, and MAL Spin Columns in the Sialic Glycoprotein Kit. [D] Eluted glycoproteins from AIL and PNA Spin Columns in the O-Glycan Glycoprotein Kit.|
Performance
The Qproteome Glycoprotein Kit allows highly specific fractionation of proteins based on their glycan moieties (see figure "Highly specific glycoprotein fractionation").
Principle

Glycosylated proteins in the sample bind to lectins immobilized on the resin in the spin columns. After washing, proteins are eluted in two stages by sequential addition of buffers containing sugars which compete with bound proteins for the lectin binding sites.

Procedure
The sample is diluted and applied to the spin column (see figure "Glycoprotein fractionation procedure"). After a short incubation, non-glycosylated proteins are removed by centrifugation. Glycosylated proteins are eluted by application of elution buffer and centrifugation.
Applications

Glycosylation plays a vital role in a wide range of cellular processes such as cell adhesion and signaling, stabilization of protein structure and function, protein trafficking and sorting, and oncogenesis. Several diseases (e.g., rheumatoid arthritis, muscular dystrophy) may be caused by a defect in protein glycosylation. The Qproteome Glycoprotein Fractionation Kit offers highly specific separation of glycoproteins according to the structure of their glycan moieties, and permits profiling of glycoproteins in cells grown under different conditions. Purified glycoproteins are suitable for direct analysis using blotting procedures, 2D-PAGE, or mass spectrometry methods.

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Feature
Specifications
Applications SDS-PAGE, mass spectrometry
Binding capacity/yield 30–150 µg
For glycoproteins: which type of glycoproteins Glycoproteins with different structures of their glycan moieties
Fractions isolated One fraction
Protein with post-translational modification Glycosylation
Sample size 50 µl or 1 x 10e7 cells
Start material Serum, cell cultures

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