Critical factors for succesful multiplex end-point PCR

Multiplex end-point PCR is a powerful tool for genotyping and many other applications. The ability to amplify and detect several DNA targets in the same reaction offers many benefits. It enables generation of more data from minimal amounts of sample material, which is especially beneficial in procedures involving limited and precious sample material. It also offers the opportunity to run internal controls in every reaction, thus increasing the overall reliability of data acquisition. Most importantly of course, switching PCR-based routine lab testing to multiplex PCR offers the opportunity to substantially save on time, materials and cost.

QIAGEN’s multiplex PCR chemistry is optimized for reliable amplification of many different templates with high variability in copy numbers. Thus, it enables very quick establishment of a new lab routine and instant success for your multiplex PCR strategy.

There are a set of critical factors that should be considered when planning and performing this type of PCR. These recommendations will be discussed in detail in the webinar. Additionally, our multiplex PCR chemistry has recently been gaining increasing popularity among scientists who are utilizing it for their next-generation sequencing workflows. We will also briefly discuss this topic.

Vishwadeepak Tripathi

Vishwadeepak Tripathi, PhD is a Global Market Manager at QIAGEN. He received his PhD in biochemistry at the Faculty of Medicine from Ruhr-University Bochum, Germany. Dr. Tripathi studied the role of chaperones and co-chaperones in protein folding and quality control and authored a number of scientific publications. He was also at RIKEN Institute in Japan where he studied the pathogenesis of Huntington's disease in cellular and mice models. He is currently interested in biomarker research, NGS and neurodegeneration.