Advanced NGS Library Prep for Challenging Samples

Dec 15, 2016 1:00 PM–2:00 PM
Duration: 1hrs

Rapidly developing next-generation sequencing (NGS) technologies provide highly sensitive methods for discovering and characterizing the genetic information of a variety of samples. However, DNA samples are often limited in quantity, as well as compromised in quality. Such samples are not suitable for standard NGS library construction methods, which commonly require hundreds of nanograms of high-quality DNA. Examples of such challenging samples include circulating DNA laser capture microdissection formalin-fixed paraffin-embedded (FFPE) samples, ancient DNA and chromatin immunoprecipitation (ChIP) samples.

In this webinar, we describe the measures that should be taken into consideration while sequencing such challenging samples. We will also present methods that can be used to optimize library construction to efficiently convert small amounts of DNA samples into high-quality sequencing libraries.

Dr. Nan Fang

Nan Fang received her Bachelor of Science in Biology from University of Science and Technology of China, Heifei, China, Ph.D. in Immunology from University of Iowa, Iowa City, Iowa, and MBA from University of Wuerzburg, Wuerzburg, Germany. She has been working at QIAGEN R&D for nine years, first on PCR and reverse transcription products and subsequently on NGS products. Nan is currently leading Life Sciences NGS product development, including products for NGS sample and library preparation and single cell applications.