Single-Cell Mutation Detection – Overcoming Challenges in Single-Cell Analysis



Genetic alteration is the driving factor behind various biological processes such as developmental malformation, evolution and cancer. Recent findings of genomic heterogeneity among supposedly homogeneous cell populations such as tumor cells demands genomic characterization at the individual cell level to better understand the underlying biology. Single nucleotide variation (SNV) analysis by next-generation sequencing (NGS) requires relatively high coverage that often necessitates targeted enrichment or amplification to focus on sites of interest.

In this webinar, we will present a multiplex PCR-based targeted enrichment sequencing method for detecting mutations in single cells isolated from two colon cancer cell lines, Lovo and HT29. We will discuss the technologies along the workflow as well as the challenges and how to address them.

Dr. Frank Narz, Director R&D Scientific Applications, QIAGEN

Frank Narz

Dr. Frank Narz is Director, R&D Scientific Applications, at QIAGEN in Hilden. His responsibilities include the development and evaluation of new assay technologies and solutions for single cell research. Dr. Narz joined QIAGEN in 2000 and has since held various positions in Research and Development in the areas of cell biology, RNA purification, Pyrosequencing and assay technologies. After studying biology, he received his Ph.D. from the Institute of Molecular Neurobiochemistry at the Faculty for Chemistry (University of Bochum, Germany) in 1999. He also completed postgraduate studies in Nanobiotechnology at the University Kaiserlautern in Germany.