A rapid, automated real-time PCR instrument for genotyping and microbial identification

Oct 24, 2017 1:00 PM–2:00 PM (EDT)
Duration: 1hrs
Molecular testing for the identification of bacterial and viral infections as well as gene mutations from raw livestock samples and medical research specimens is always a challenging task. It requires a multistep approach to optimize assays and to eliminate inhibitory effects in PCR amplification. Laboratory benchtop PCR assay setup procedures can be very labor intensive and unreliable, which is reflected in its prolonged assay time, poor reproducibility and increasing total assay costs. The need for automation has led to the development and introduction of robotic laboratory instruments, aiming to decrease operator errors and process time, and to increase process safety.

We have developed a selection of robust, novel chemistries to prevent PCR crosstalk. We can successfully measure target abundance and fold change in real-time assays, and perform sub-genotyping using a fast, high-throughput and powerful High-Resolution Melting (HRM) statistical analysis program. We have also tested a liquid handler for quick PCR assay setup to eliminate manual steps and increase productivity. In this presentation, we will demonstrate these features and benefits with examples.

James Qin

James is a senior scientist at QIAGEN and has graduate degrees in Molecular and Cell Biology and Computer Science. For the past 14 years, James has been a key member in developing siRNA assays and microRNA detection products. Prior to joining QIAGEN, James has accumulated over 10 years of experience in the biotech industry in library construction, screening, cell culture and transfection, biomarker content development, array and assay technology development, DNA micro array analysis and gene expression data mining.