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GeneRead Size Selection Kit (50)
For 50 reactions: Spin columns and buffers
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180514
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The GeneRead Size Selection Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Precise size selection.|
The GeneRead Size Selection Kit effectively removes adapter dimers and adapter monomers following library preparation. A scaled-up image of the above data showing the correct size distribution of Illumina-compatible library fragments following size selection is shown in inset. FU: Fluorescence units.|
Performance
Fast and precise size selection
The GeneRead Size Selection Kit uses a convenient, spin-column-based procedure to ensure precise size selection of the DNA library, while effectively removing adapter dimers or monomers (see figure Precise size selection). DNA fragments shorter than 150 bp are reliably removed.
Principle
The GeneRead Size Selection Kit combines the convenience of spin-column technology with the selective properties of a uniquely designed binding buffer. MinElute spin columns provided with the kit ensure high concentrations of purified DNA for subsequent reactions. The special buffer provided with the kit is optimized for efficient removal of small DNA fragments such as adapters and adapter dimers. Larger DNA fragments adsorb to the silica membrane in the presence of optimized concentrations of salt, while contaminants and smaller fragments pass through the column. This leads to a cut-off limit (defined as the length of the shortest fragment that can be visualized by electropherogram) of 150 bp. Impurities such as enzymes from previous reactions are efficiently washed away, and pure and size-selected DNA is eluted. For optimal adapter monomer and dimer removal, together with high DNA recovery, two subsequent purification steps are performed for size selection during library preparations for next-generation sequencing.
Procedure
The GeneRead Size Selection Kit uses a convenient, spin-column-based procedure to ensure precise size selection of the DNA library, while effectively removing adapter dimers or monomers. Automatable on the QIAcube, the easy-to-use protocol saves time by eliminating tedious handling procedures, and ensures there is no risk of carryover of potentially inhibitory ethanol or beads (see figure
Precise size selection).
Applications
The GeneRead Size Selection Kit enables quick and reliable removal of DNA fragments shorter than 150 bp for subsequent use in library preparation in NGS.