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DNeasy PowerWater Sterivex Kit

For the isolation of genomic DNA from all filtered water sample types collected with Sterivex filter units

Features

  • In-unit digestion saves time and samples
  • Column-based purification protocol eliminates the need for harsh chemicals
  • Fast protocol takes only 40 minutes
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DNeasy PowerWater Sterivex Kit (50)

Cat. No. / ID: 14600-50-NF

For the isolation of genomic DNA from all filtered water sample types collected with Sterivex filter units
CA$1,031.00
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The DNeasy PowerWater Sterivex Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

Removing important sample data from Millipore’s Sterivex filter units present a unique set of problems for researchers, however the DNeasy PowerWater Sterivex Kit is the only kit available to provide DNA from these filters without enzymes or harsh organic chemicals. Microbes are released from Sterivex filter units utilizing a novel Cell Release Solution that eliminates extensive incubation times or breaking the casing around the membrane. With Inhibitor Removal Technology, high-quality DNA isolation is possible, even when the original sample was highly contaminated. In 40 minutes, DNA can be purified from all types of water samples.

This kit is recommended for use with Millipore catalog # SVGPL10RC Sterivex GP Filter units, but is also compatible with other Sterivex Filter units.

The DNeasy PowerWater Sterivex Kit was previously sold by MO BIO as the PowerWater Sterivex DNA Kit.

Procedure

To begin the DNeasy PowerWater Sterivex Kit protocol, Sterivex units are treated and incubated with cell release solution. Units are then treated with lysis buffer, followed by mixing and removing lysate for homogenization in a 5 ml bead beating tube. Total genomic DNA is captured after protein and inhibitor removal steps on a silica binding column using a vacuum manifold. The binding column membrane is then washed, resulting in pure, high-quality DNA being eluted for downstream applications.

Supporting data and figures

Resources

Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.
Quick-Start Protocols (1)

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699