QIAamp 96 DNA Swab BioRobot Kit

For automated high-throughput DNA purification from swabs

Features

  • Rapid purification of high-quality DNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Removal of contaminants and inhibitors

Useful Resources

QIAamp 96 DNA Swab BioRobot Kit (12)

Cat. No. / ID: 965842

For 12 x 96 DNA preps: 12 QIAamp 96 Plates, Buffers, QIAGEN Proteinase K, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), Caps
$4,324.00
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The QIAamp 96 DNA Swab BioRobot Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

The QIAamp 96 DNA Swab BioRobot Kit provides automated DNA purification from up to 192 swabs on the BioRobot Universal System using proven QIAamp silica-membrane technology. Up to 192 samples are processed in less than 2.5 hours. The procedure is optimized for use with air-dried swabs with plastic shafts and cotton or DACRON tips, although other swab types can be used.

Performance

Proven QIAamp 96 technology provides well-to-well uniformity in DNA recovery and purity with no cross-contamination between samples (see figure " Cross-contamination test"). The effective removal of enzyme inhibitors ensures reliable performance in downstream applications such as TaqMan®, LightCycler, and iCycler analyses (see figure " Reliable performance").

DNA isolated with the QIAamp 96 DNA Swab BioRobot Kit is ready to use in a wide range of applications in molecular diagnostics and forensic science:

See figures

Principle

No phenol–chloroform extraction is required. DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in four efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp technology yields DNA from swabs ready to use in PCR and blotting procedures.

Procedure

The QIAamp 96 DNA Swab BioRobot Kit simplifies isolation of DNA from swabs with a fast automated 96-well–plate procedure (see flowchart " QIAamp 96 DNA Swab BioRobot procedure"). The procedure is optimized for use with air-dried swabs with plastic shafts and cotton or DACRON tips, although other swab types can be used. The typical yield for the QIAamp 96 DNA Swab BioRobot Kit is 1–2 µg per swab (buccal swabs), with an elution volume of 150 µl.

QIAamp sample preparation technology is fully licensed.

See figures

Applications

The QIAamp 96 DNA Swab BioRobot Kit provides QIAamp technology in a convenient 96-well format for high-throughput purification needs. The DNA from up to 96 swab samples is purified in under 2 hours. Sample types include:

  • Buccal swabs
  • Pharyngeal swabs
  • Eye swabs

Supporting data and figures

Specifications

FeaturesSpecifications
applicationsPCR, blotting
yield1–2 µg per swab
format96-well plate
forautomatedprocessingBioRobot Universal System, BioRobot Genotyping, BioRobot 9604
sampleamount1 swab per well
processingAutomated
elutionvolume150 µl
purificationoftotalrnamirnapolyamrnadnaorproteinGenomic DNA
mainsampletypeSwabs
technologySilica technology
timeperrunorperprep<2 hours

Resources

Kit Handbooks (1)
For automated purification of genomic DNA from buccal swabs using the BioRobot Universal System, BioRobot Genotyping, or BioRobot 9604

Publications

Estrogen sulfation genes, hormone replacement therapy, and endometrial cancer risk.
Rebbeck TR; Troxel AB; Wang Y; Walker AH; Panossian S; Gallagher S; Shatalova EG; Blanchard R; Bunin G; DeMichele A; Rubin SC; Baumgarten M; Berlin M; Schinnar R; Berlin JA; Strom BL;
J Natl Cancer Inst; 2006; 98 (18):1311-20 2006 Sep 20 PMID:16985250
Isolation of genomic DNA from buccal swabs for forensic analysis, using fully automated silica-membrane purification technology.
Hanselle T; Otte M; Schnibbe T; Smythe E; Krieg-Schneider F;
Leg Med (Tokyo); 2003; 5 Suppl 1 :S145-9 2003 Mar PMID:12935575

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699
What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

For more information on activity of QIAGEN Protease and Proteinase K in various buffers please click here.

FAQ ID -761
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12