DNeasy PowerFood Microbial Kit

For the isolation of inhibitor-free DNA from a variety of cultured foods

Features

  • Simplified isolation of purified DNA from a variety of food types, pathogens, yeasts and fungi
  • Efficient removal of PCR inhibitors including lipids and polysaccharides
  • High-quality, ready-to-use DNA for downstream applications
  • Streamlined protocol can isolate DNA from food cultures or uncultured food
DNeasy PowerFood Microbial Kit (100)

Cat. No. / ID: 21000-100

For the isolation of inhibitor-free DNA from a variety of cultured foods
CA$605.00
Size
0.5 ml
2 ml
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The DNeasy PowerFood Microbial Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Isolating high-quality genomic DNA from tough, food cultured microorganisms is easy with the DNeasy PowerFood Microbial Kit. As a complete kit, no additional reagents and enzymes are needed to process vital samples. The Inhibitor Removal Technology incorporated into the kit removes PCR-inhibiting compounds associated with food cultures, including lipids and polysaccharides. Processed DNA is ready for use in downstream applications including qPCR and genotyping.

The DNeasy PowerFood Microbial Kit has been validated with a wide range of food products including meats, dairy products, fruits, vegetables and juices, isolating PCR inhibitor free DNA from pathogens including Clostridium difficile, Clostridium perfringens, Enterococcus faecalis, Escherichia coli, Lactobacillus sp., Listeria monocytogenes and Salmonella enterica.

The DNeasy PowerFood Microbial Kit is available with either 0.5 ml bead tubes containing 0.15 mm garnet beads (21000-100) or with 2 ml bead tubes containing 0.15 mm garnet beads (21000-100-MON). The 0.5 ml bead tubes are best for DNA isolation from food cultures, while the 2 ml bead tubes work best for isolation of DNA from uncultured food. Want to try this solution for the first time? Request a quote for a trial kit.

The DNeasy PowerFood Microbial Kit was previously sold by MO BIO as the PowerFood Microbial DNA Kit.

Specifications

FeaturesSpecifications
formatSilica Spin Filter Tubes
samplesize1.8 ml cultured food or 0.25 g of food material
throughput1-24 samples
bindingcapacityUp to 20 µg per prep
storagetemperatureStore at room temperature (15-30°C)
sampletypesProcessed cultured foods, dairy (milk, yogurt, cheese), juices, vegetables, fruits, chocolate, washes
timeperrunorperprep25 minutes
beadsize0.15 mm garnet

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699