DNase Max Kit

For the removal of genomic DNA contamination in RNA preparations

Features

  • High-activity DNase I enzyme maintains long term stability at room temperature
  • Efficient removal of DNase I enzyme without the need for heat-treating or EDTA
  • Optimal qPCR results with complete removal of DNase and divalent cations
  • Short, 30-minute protocol from start to finish
DNase Max Kit (50)

Cat. No. / ID: 15200-50

For the removal of genomic DNA contamination in RNA preparations
CA$178.00
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The DNase Max Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Removing genomic DNA contamination from RNA preparations is easy with the enzyme-resin combination in the DNase Max Kit. The highly purified DNase I enzyme is formulated for long term stability at room temperature. This proprietary enzyme removes up to 30 µg of DNA in 20 minutes and is shelf stable for up to 6 months at room temperature. At 4°C the enzyme will remain stable for up to 2 years without loss of activity. Additionally, the DNase Max Kit contains a novel resin to bind and remove the DNase Max enzyme and divalent cations from the reaction, eliminating the need for heat-treating or EDTA inactivation of the DNase.

Resulting RNA is ready to use in downstream applications immediately after resin treatment.

The DNase Max Kit was previously sold by MO BIO under the same name.

Performance



Supporting data and figures

Specifications

FeaturesSpecifications
scale10 units/µl, removes 30 µg DNA
time30 minutes
storagetemperatureStore at room temperature (15-30°C) for up to 6 months or at 4°C until the expiration date

Resources

Kit Handbooks (1)
DNase Max Handbook
PDF (105KB)
Quick-Start Protocols (1)

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699