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MinElute 96 UF PCR Purification Kit

For high-throughput, ultrafiltration purification of up to 15 μg PCR products (≥100 bp)

Features

  • Very small elution volumes
  • Fast procedure and easy handling
  • High, reproducible recoveries
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MinElute 96 UF PCR Purification Kit (24)

Cat. No. / ID: 28053

24 MinElute 96 UF PCR Purification Plates
CA$1,528.00
Pieces
24
4
Add to cart
The MinElute 96 UF PCR Purification Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

The MinElute 96 UF PCR Purification Kit provides 96-well plates for high-throughput ultrafiltration-based purification of PCR products ≥100 bp in size. The plates are designed to allow elution in very small volumes (as little as 20 μl) delivering high yields of highly concentrated DNA. The procedure can be automated on the BioRobot Universal System. DNA fragments purified with the MinElute system are ready for direct use in many applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription. The MinElute 96 UF PCR Purification Kit requires the use of the QIAvac Multiwell vacuum manifold (cat. no. 9014579).

The product must be used in conjunction with QIAvac Multiwell.

Performance

The MinElute 96 UF PCR Purification Kit provides high-throughput PCR purification in 96-well plates. Fully automated processing is provided for 1–8 x 96 samples on the BioRobot 3000 (no longer available), the BioRobot 8000, or the BioRobot Universal System, or for 1 x 96 samples on the QIAvac Multiwell Manifold (cat. no. 9014579, requires suitable vacuum manifold, e.g., QIAvac Multiwell manifold, plate shaker recommended). The procedure purifies DNA ≥100 bp in size with a recovery of 80–95%. Purified PCR products are eluted directly from the surface of the membrane in small volumes (as little as 20 µl in the manual procedure or 30 µl on BioRobot workstations), providing high-concentration eluates (see figure " Highly concentrated DNA eluates") that are suitable for direct use in a variety of downstream applications.  It is possible to process PCR sample volumes up to 150 µl using either a manual or fully automated procedure.

See figures

Principle

The QIAGEN MinElute 96 UF PCR Purification Kit combines the convenience of multiwell technology and the separation properties of an ultrafiltration membrane with a unique design. The plates enable the fast and efficient recovery of double-stranded DNA fragments ≥100 bp and the removal of primers (<20mers), unincorporated nucleotides, and salts.

Procedure

MinElute 96 UF PCR Purification Kits provide ultrafiltration technology using vacuum-driven purification on QIAvac manifolds (see flowchart " MinElute 96 UF PCR Purification procedure"). PCR products are loaded into the wells of the ultrafiltration plates, and a vacuum is applied. While small molecules such as primers, salts, and unincorporated nucleotides pass through the membrane, PCR products ≥100 bp are retained. Water, DMSO, or buffers such as SSC can be used for elution. The MinElute 96 UF PCR Purification procedure can also be automated on the BioRobot 3000 (no longer available), the BioRobot 8000, or the BioRobot Universal System.

See figures

Applications

DNA fragments purified with the MinElute 96 UF system are ready for direct use in many applications, including:

  • Sequencing
  • Microarray analysis
  • Ligation and transformation
  • Restriction digestion
  • Labeling

Supporting data and figures

Specifications

FeaturesSpecifications
Binding capacity<150 µl
Recovery: oligonucleotides dsDNARecovery: oligonucleotides, dsDNA
Elution volume20–30µl
Format96-well plate
Fragment size>100 bp
Removal <10mers 17–40mers dye terminator proteinsRemoval <20mers
TechnologyUltrafiltration
Sample type: applicationsDNA, oligonucleotides: PCR reactions
ProcessingManual/automated

Resources

Kit Handbooks (1)
For efficient, high-throughput purification of PCR products
Quick-Start Protocols (1)

Publications

Genome-wide analysis of DNA copy number changes and LOH in CLL using high-density SNP arrays.
Pfeifer D; Pantic M; Skatulla I; Rawluk J; Kreutz C; Martens UM; Fisch P; Timmer J; Veelken H;
Blood; 2006; 109 (3):1202-10 2006 Oct 19 PMID:17053054
Genomic and functional profiling of duplicated chromosome 15 cell lines reveal regulatory alterations in UBE3A-associated ubiquitin-proteasome pathway processes.
Baron CA; Tepper CG; Liu SY; Davis RR; Wang NJ; Schanen NC; Gregg JP;
Hum Mol Genet; 2006; 15 (6):853-69 2006 Jan 30 PMID:16446308
Multilocus sequence typing of Staphylococcus aureus isolated from high-somatic-cell-count cows and the environment of an organic dairy farm in the United Kingdom.
Smith EM; Green LE; Medley GF; Bird HE; Dowson CG;
J Clin Microbiol; 2005; 43 (9):4731-6 2005 Sep PMID:16145134
Multilocus sequence typing of intercontinental bovine Staphylococcus aureus isolates.
Smith EM; Green LE; Medley GF; Bird HE; Fox LK; Schukken YH; Kruze JV; Bradley AJ; Zadoks RN; Dowson CG;
J Clin Microbiol; 2005; 43 (9):4737-43 2005 Sep PMID:16145135
Pooled association genome scanning: validation and use to identify addiction vulnerability loci in two samples.
Liu QR; Drgon T; Walther D; Johnson C; Poleskaya O; Hess J; Uhl GR;
Proc Natl Acad Sci U S A; 2005; 102 (33):11864-9 2005 Aug 9 PMID:16091475

FAQ

Can MinElute 96 UF PCR be used to purify PCR samples which contain mineral oil?
We do not recommend the use of samples containing mineral oil since mineral oil will not be removed during the purification procedure.
FAQ ID -360
Can I use a partial MinElute 96 UF plate?

Yes, you may use part of the MinElute 96 UF Plate, and use the rest of the plate later. In contrast to silica membrane based plates, the unused part of the UF plate does not need to be taped off. We do not recommend to re-use MinElute 96 UF Plates more than 2-4 times.

 

FAQ ID -355
Do you have a protocol for concentration of RNA in 96-well plates?

Yes, please follow the User-Developed Protocol 'Concentration of RNA in 96-well plates using the MinElute 96 UF PCR Purification Kit' (RN03).

 

FAQ ID -1546