The artus BK Virus RG PCR Kit and the artus BK Virus QS-RGQ Kit are based on the amplification and simultaneous detection of a specific region of the BK virus genome using real-time PCR for research applications.
Each artus BK Virus Kit provides 4 BK virus quantitation standards (see figure " Quantitation of BK virus DNA"). Use of the standards enables quantitation of BK virus DNA. In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical BK virus PCR is not reduced.
|Kit||artus BK Virus RG PCR Kit and artus BK Virus QS-RGQ Kit|
|Sample types||Plasma or urine|
|Amplicon||274 bp region covering parts of the VP2 and VP3 genes|
artus BK Virus Kits provide all necessary reagents optimized for reliable BK virus DNA detection and quantitation. Simply add template DNA to the ready-to-use PCR master mix and Mg solution, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.
The QIAsymphony RGQ workflow solution for BK virus detection comprises the QIAsymphony SP for sample preparation, the QIAsymphony AS for assay setup, and the artus BK Virus QS-RGQ Kit on the Rotor-Gene Q. The system enables reliable pathogen detection with a complete workflow (see figure " Integrated QIAsymphony RGQ system for BK virus detection").
For the artus BK Virus RG PCR Kit, we recommend using the EZ1 DSP Virus Kit.
The artus BK Virus RG PCR Kit enables rapid and sensitive detection of BK virus DNA from plasma or urine samples with highly accurate quantification on Rotor-Gene Q instruments for research applications.
The artus BK Virus QS-RGQ Kit is designed to be used with the QIAsymphony RGQ system, providing a complete workflow from plasma or urine samples to BK virus detection and quantitation.