QIAamp DSP Virus Kit

For purification of viral nucleic acids from human plasma and serum for in vitro diagnostic use

Features

  • Universal purification system compatible with other IVD products
  • Hiqh-quality viral nucleic acids
  • Concentration with 20 µl or 60 µl eluates
  • Sample volumes of 500 µl
  • Rapid purification and minimal risk of cross-contamination
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QIAamp DSP Virus Kit

Cat. No. / ID: 60704

For 50 preps: QIAamp MinElute Columns, buffers, reagents, tubes, column extenders, VacConnectors
CA$365.00
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The QIAamp DSP Virus Kit is intended for in vitro diagnostic use.

Product Details

The QIAamp DSP Virus Kit uses well-established and convenient QIAamp technology for simultaneous purification of viral DNA and RNA for in vitro diagnostic use.

Performance

Viral nucleic acids purified using the QIAamp DSP Virus Kit are ready to use in sensitive downstream applications, such as those based on enzymatic amplification or other modification, including PCR and RT-PCR.

Principle

The QIAamp DSP Virus Kit uses well-established and convenient QIAamp technology for simultaneous purification of viral DNA and RNA. The QIAamp silica-based membrane binds nucleic acids in the lysed sample, while the rest of the lysate is rapidly removed by vacuum pressure. The bound nucleic acids are efficiently washed to remove contaminants and then eluted in a volume of 20 µl or 60 µl.

Procedure

The QIAamp DSP Virus Kit Procedure includes the following steps: lyse, bind, wash (3x), dry spin, and elute.  Viral nucleic acids are purified from plasma or serum using a vacuum manifold, such as the QIAvac 24 Plus (see flowchart, " Procedure").
See figures

Applications

Viral nucleic acids can be purified from plasma or serum samples. Samples can contain the anticoagulants citrate or EDTA, and can be either fresh, lyophilized, or frozen (provided they were not thawed and refrozen).

The QIAamp DSP Virus Kit provides purification of nucleic acids from a wide range of viruses. The kit is compatible with a wide range of upstream sample collection systems and downstream applications, and therefore can be easily integrated into diagnostic workflows.

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsPCR, RT-PCR, LCR
Elution volume20 µl, 60 µl
Main sample typeSerum, plasma
CE/FDA/IVD compatibleCE/IVD
FormatSample tubes
ProcessingManual (vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinViral DNA, viral RNA
Sample amount500 µl
TechnologySilica technology
Time per run or per prep25 minutes
YieldVaries

Resources

Kit Handbooks (1)
QIAamp DSP Virus Kit Handbook
Brochures & Guides (1)

Publications

Occurrence of hepatitis A virus genotype III in Germany requires the adaptation of commercially available diagnostic test systems.
Heitmann A; Laue T; Schottstedt V; Dotzauer A; Pichl L;
Transfusion; 2005; 45 (7):1097-105 2005 Jul PMID:15987353

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699
What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

For more information on activity of QIAGEN Protease and Proteinase K in various buffers please click here.

FAQ ID -761
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728
What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer?

For RNA isolated on the BioRobot EZ1 and BioRobot M48:

The RNA can be directly applied to the Agilent Bioanalyzer, since it is being denatured in the final protocol steps of these isolation procedures.

For RNA prepared with all other QIAGEN RNA Isolation Products:

We recommend to denature the samples in a water bath for 2 min at 70°C, and then place them directly on ice prior to loading them onto the Agilent Bioanalyzer.

 

FAQ ID -528
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12