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QIAGEN Proteinase K

For protease digestion during DNA and RNA preparation

    QIAGEN Protease and QIAGEN Proteinase K offer broad substrate specificity with high activity for a wide range of reaction conditions. Both proteases offer high activity in buffers commonly used in most DNA and RNA isolation procedures and are quality-guaranteed by QIAGEN.

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    Cat No./ID: 19131
    QIAGEN Proteinase K (2 ml)
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    2 ml (>600 mAU/ml, solution)
    Cat No./ID: 19133
    QIAGEN Proteinase K (10 ml)
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    10 ml (>600 mAU/ml, solution)

    Product Details

    Performance

    QIAGEN Protease and QIAGEN Proteinase K offer broad substrate specificity with high activity in buffers commonly used in most DNA and RNA isolation procedures as well as in a wide range of salt, denaturant, and detergent (see table "Protease activity in commonly used buffers"), pH, and temperature conditions. Both enzymes are quality-guaranteed by QIAGEN.

    Protease activity in commonly used buffers*
    Buffer Activity (%)
    QIAGEN
    Protease    		 QIAGEN
    Proteinase K
    30 mM Tris·Cl 100 100
    30 mM Tris·Cl; 30 mM EDTA; 5% Tween 20; 0.5% Triton X-100; 800 mM GuHCl† 210 313
    36 mM Tris·Cl; 36 mM EDTA; 5% Tween 20; 0.36% Triton X-100; 735 mM GuHCl‡ 205 301
    10 mM Tris·Cl; 25 mM EDTA; 100 mM NaCl; 0.5% SDS 78 128
    10 mM Tris·Cl; 100 mM EDTA; 20 mM NaCl; 1% Sarkosyl 101 74
    10 mM Tris·Cl; 50 mM KCl; 1.5 mM MgCl2; 0.45% Tween 20; 0.5% Triton X-100 159 106
    10 mM Tris·Cl; 100 mM EDTA; 0.5% SDS 98 120
    30 mM Tris·Cl; 10 mM EDTA; 1% SDS 38 203
    * pH 8.0, 50°C, 1.25 µg/ml protease, 15 min incubation.
    † Buffer G2 used in QIAGEN Genomic-tip procedures for DNA isolation from blood, cell cultures, and tissue.
    ‡ Recommended buffer conditions for protease digestion (Buffers B1+ B2) in the QIAGEN Genomic-tip protocol for bacterial DNA isolation.
    Principle
    Technical specifications
    QIAGEN Protease QIAGEN Proteinase K
    Format Lyophilized powder Ready-to-use solution
    Amount 7.5 AU or 4 x 7.5 AU 2 ml or 10 ml (20 mg/ml)
    Activity 45 mAU/mg protein >318 mAU/ml (30°C)
    Unit definition One mAU is the activity that releases folin-positive amino acids and peptides corresponding to 1 µmol tyrosine per minute

    QIAGEN Proteinase K is a subtilisin-type protease isolated from the saprophytic fungus Tritirachium album and is particularly suitable for short digestion times. It possesses a high specific activity which remains stable over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity. Proteinase K is supplied in the following QIAGEN kits:

    QIAamp DNA Mini Kit
    QIAamp DNA Stool Mini Kit
    QIAamp 96 DNA Swab BioRobot Kit
    DNeasy Tissue Kit
    DNeasy 96 Tissue Kit
    QIAamp DNA Micro Kit
    QIAamp MinElute Media Kit
    QIAamp UltraSens Virus Kit
    QIAamp Media MDx Kit

    QIAGEN Protease is a serine protease isolated from a recombinant Bacillus strain and is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of sources. QIAGEN Protease is completely free of DNase and RNase activities. QIAGEN Protease is supplied in the following QIAGEN kits:

    QIAamp DNA Blood Mini, Midi, and Maxi Kits
    QIAamp 96 DNA Blood Kit
    Blood & Cell Culture DNA Kits
    QIAamp DNA Blood BioRobot 9604 Kit
    QIAamp Virus BioRobot 9604 Kit
    QIAamp DNA Blood BioRobot MDx Kit
    QIAamp DSP 96 Virus MDx Kit
    QIAamp DSP Virus Kit
    QIAamp DSP DNA Blood Mini Kit

    Note: Users of manual QIAamp DNA Blood Kits and QIAGEN Blood & Cell Culture DNA Kits should resuspend each bottle of QIAGEN Protease with 7 ml distilled water.

    Note: Users of the QIAamp DNA Blood BioRobot 9604 Kit should resuspend each bottle of QIAGEN Protease with 10 ml distilled water.

    Note: QIAGEN Protease is not compatible with Buffer ATL in DNeasy Tissue, DNeasy 96 Tissue, and the QIAamp DNA Mini Kit. In the presence of >0.5% SDS, >1% sarkosyl, or high concentrations of other detergents, the EDTA concentration must be <8 mM for full activity over extended incubation times.

    Procedure

    Instructions for using QIAGEN Protease or QIAGEN Proteinase K are provided in the corresponding kit handbook.

    Applications

    QIAGEN Protease and QIAGEN Proteinase K provide protease digestion during DNA and RNA preparation. Subtle differences between the enzymes should be considered when planning protease digestions.

    Product Resources

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    User-Developed Protocols (2)
    DNA extraction from fired and unfired ammunition using the EZ1 DNA Investigator Kit - (EN)
    This protocol has been adapted by the San Diego Police Department (SDPD) Crime Laboratory from the standard QIAGEN pretreatment for casework samples, and is intended for the lysis and extraction of DNA from forensic samples of fired (casings) and unfired (cartridges) ammunition.
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    Isolation of DNA from tofu using the DNeasy® Plant Mini Kit - (EN)

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    Product citations in scientific articles

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