Get 5% off the enzyme of your choice.
Use the code ENZ2526 at checkout. Offer valid from December 1, 2025, to March 31, 2026. T&Cs* apply.
RNase A
Cat no. / ID. 19101
2.5 ml (100 mg/ml; 7000 units/ml, solution)
*Offer valid on orders placed from December 1, 2025, to March 31, 2026, or while stocks last. Offer void where prohibited and cannot be combined with any other promotion. Offer is only valid in the following countries: Australia, Austria, Belgium, Canada, Denmark, Finland, France, Germany, Italy, Luxembourg, Netherlands, New Zealand, Norway, Poland, South Africa, Sweden, Switzerland, United Kingdom, United States of America. Freight and delivery costs are not included. It is the customer's responsibility to ensure that acceptance of this offer will not violate any internal policies of the customer's organization and applicable laws and regulations.
The RNase A is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Get 5% off the enzyme of your choice.
Use the code ENZ2526 at checkout. Offer valid from December 1, 2025, to March 31, 2026. T&Cs* apply.
Features
- Selectively cleaves single-stranded RNA
- Active under a wide range of reaction conditions
- DNase-free and quality-controlled
- Does not need to be boiled before use
Product Details
RNase A is an endoribonuclease that breaks down single-stranded RNA molecules by hydrolysis 3' of pyrimidine residues (cytosine and uracil). RNase A is a basic protein of 124 amino acids, with a molecular weight of approximately 13.7 kDa. RNase A catalyzes the transphosphorylation and degradation of RNA by cleaving specifically at several sites on a single stranded RNA polynucleotide chain at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds. Ribonucleases like RNase A do not hydrolyze DNA because the DNA lacks 2′-OH groups essential for forming cyclic intermediates. RNase A is widely used to remove contaminating RNA during the isolation of plasmid and genomic DNA.
DNase-free Ribonuclease A is quality-controlled and ready to use for all applications where digestion of RNA is required.
Performance
QIAGEN Ribonuclease A is DNase-free and quality-controlled for use in plasmid purification procedures for the digestion of RNA.
RNase A is widely used to remove contaminating RNA during the isolation of plasmid and genomic DNA. RNase A is also used to remove contaminating RNA from DNA samples after extraction or PCR, especially if downstream applications (e.g., cloning or sequencing) require RNA-free DNA.
RNase A is very active under a wide range of reaction conditions and is difficult to inactivate.
QIAGEN RNase A does not need to be boiled before use.
| Feature | RNase A |
|---|---|
| Quantity | 2.5 mL |
| Concentration | 100 mg/; 7000 units/ml, solution |
| Unit definition | One Kunitz unit is the amount of enzyme causing the hydrolysis of RNA at a rate such that the velocity constant (k) equals unity at 25°C and pH 5.0 |
Procedure
Before use in plasmid and DNA purification, centrifuge QIAGEN RNase A briefly, and then add into Buffer P1 to obtain a final concentration of 100 μg/mL. Store Buffer P1 at 2–8°C after addition of RNase A. For detailed instructions on RNase A usage during plasmid and DNA purification, please refer to the corresponding kit handbook.
Applications
- Plasmid and genomic DNA preparation
- Removal of RNA contamination from recombinant protein preparations
- Cleanup of RNA from enzymatic reactions
Resources
Safety Data Sheets (1)
Brochures & Guides (1)
Certificates of Analysis (1)
FAQ
3314-What is the concentration of RNase A sold separately?