QIAamp MinElute Virus Spin Kit
For simultaneous purification of viral DNA and RNA from plasma, serum, and cell-free body fluids
- Rapid purification of high-quality viral DNA and RNA
- No organic extraction or alcohol precipitation
- Consistent, high yields
- Complete removal of contaminants and inhibitors
The QIAamp MinElute Virus Spin Kit simplifies purification of viral DNA and RNA with fast spin-column procedures. The QIAamp MinElute Virus Spin Kit uses starting sample volumes of up to 0.2 ml and combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 μl. The QIAamp MinElute Virus Spin process can be fully automated on the QIAcube Connect.
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QIAamp MinElute Virus Spin Kit (50)
For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, carrier RNA, buffers, Collection Tubes (2 ml)
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57704
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QIAamp MinElute Virus Spin Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。
QIAamp MinElute Virus Spin procedure.|High sensitivity in PCR.|High sensitivity in RT-PCR.|
|The percentage of positive samples at low virus titers is shown. Nucleic acids from a DNA virus were purified from plasma using either the QIAamp MinElute Spin or Vacuum Kit and subjected to PCR. For the spin procedure, 95% probit values for 40 samples were 27.25 IU/ml and for the vacuum procedure, they were 9.30 IU/ml.|The percentage of positive samples at low virus titers is shown. Nucleic acids from an RNA virus were purified from plasma using either the QIAamp MinElute Spin or Vacuum Kit and subject to RT-PCR. For the spin procedure, 95% probit values for 32 samples were 22.84 IU/ml and for the vacuum procedure, they were 9.46 IU/ml. |
Performance
Purified viral nucleic acids are free of proteins, nucleases, and other impurities, and suitable for use in sensitive downstream applications such as PCR and RT-PCR (see figures "High sensitivity in PCR" and "High sensitivity in RT-PCR").
Viral nucleic acids purified using the QIAamp MinElute Virus Spin Kit can be used in a wide range of downstream applications, including:
- PCR and quantitative real-time PCR
- Infectious disease research
Principle
The QIAamp MinElute Virus Spin Kit simplifies isolation of viral RNA and DNA from plasma, serum, and cell-free body fluids with a fast spin-column procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp MinElute silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit. QIAamp MinElute technology yields viral RNA and DNA from plasma, serum, and cell-free body fluids ready to use in PCR and blotting procedures.
The QIAamp MinElute Spin Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 µl. The procedure is suitable for use with plasma, serum, and other cell-free body fluids. QIAamp sample preparation technology is fully licensed.
Procedure
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp MinElute membrane (see flowchart " QIAamp MinElute Virus Spin procedure"). Alcohol is added and lysates loaded onto the QIAamp MinElute spin column. Wash buffers are used to remove impurities and viral nucleic acids are eluted in Buffer AVE, ready for use in amplification reactions or storage at –20ºC. Purified nucleic acids are free of proteins, nucleases, and other impurities.
Applications
The QIAamp MinElute Virus Spin Kit uses well-established technology for simultaneous purification of viral RNA and DNA from:
- Fresh or frozen plasma
- Serum
- Other cell-free body fluids
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特点
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参数
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应用
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PCR, real-time PCR
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洗脱体积
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20–150 µl
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规格
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MinElute columns
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主要样本类型
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Serum, plasma
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处理
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Manual (centrifugation)
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纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白
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Viral DNA, viral RNA
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样本量
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200 µl
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技术
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Silica technology
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每次运行或制备时间
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<1 hour
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产量
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Varies
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FAQ ID -295
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浏览
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FAQ ID -351
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FAQ ID -472
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FAQ ID -635
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FAQ ID -761
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FAQ ID - 3560
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FAQ ID -528
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FAQ ID -728
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浏览
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FAQ ID -12
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浏览
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Download Safety Data Sheets for QIAGEN product components.
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图片
QIAamp MinElute Virus Spin实验流程。
High sensitivity in PCR.
The percentage of positive samples at low virus titers is shown. Nucleic acids from a DNA virus were purified from plasma using either the QIAamp MinElute Spin or Vacuum Kit and subjected to PCR. For the spin procedure, 95% probit values for 40 samples were 27.25 IU/ml and for the vacuum procedure, they were 9.30 IU/ml.
RT-PCR的高灵敏度。
低病毒滴度的阳性样本百分比如图所示。使用QIAamp MinElute Spin或Vacuum Kit从血浆中纯化RNA病毒核酸,并进行RT-PCR。使用离心柱步骤纯化的32个样本的95%概率值为22.84 IU/ml,使用真空步骤的95%概率值为9.46 IU/ml。(数据由QIAGEN R&D部门生成。)
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