MinElute Reaction Cleanup Kit

For cleanup of up to 5 μg DNA (70 bp to 4 kb) from enzymatic reactions
  • Very small elution volumes
  • Fast procedure and easy handling
  • High, reproducible recoveries
  • Gel loading dye for convenient sample analysis

The MinElute Reaction Cleanup Kit provides spin columns, buffers, and collection tubes for silica membrane-based purification of DNA 70 bp – 4 kb in size from enzymatic reactions. The spin columns are designed to allow elution in very small volumes (as little as 10 μl), delivering highly concentrated DNA in high yields. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube Connect. DNA fragments purified with the MinElute system are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription.
For optimal results it is recommended to use this product together with QIAvac 24 Plus.

产品 货号 目录价:
MinElute Reaction Cleanup Kit (50)
50 MinElute Spin Columns, Buffers, Collection Tubes (2&nbsp:ml)
28204
询价
MinElute Reaction Cleanup Kit (250)
250 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
28206
询价

MinElute Reaction Cleanup Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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MinElute实验流程。|GelPilot Loading Dye。|MinElute过滤膜的组成。|pH指示剂染料。|离心柱处理选择 — D。|离心柱处理选择 — E。|离心柱处理选择 — C。|离心柱处理选择 — B。|离心柱处理选择 — A。|
简单的结合-洗涤-洗脱流程十分便利。|GelPilot Loading Dye含有3种示踪染料,有助于优化DNA分辨率。|MinElute离心柱的横断面显示了独特的膜组成。|利用溶解液和结合缓冲液中的pH指示剂染料可轻松观测DNA吸附的最佳pH值 (pH ≤7.5)。如果琼脂糖凝胶电泳缓冲液使用频繁或者配制不当,则会出现错误的结合混合物pH值。此时,可加入10 µl 3 M醋酸钠(pH 5.0)轻松调整pH值。|QIAvac 24 plus。|QIAcube全自动核酸纯化仪。|带有鲁尔接头的底座。|QIAvac 24。|微型离心机。|
性能

The MinElute Reaction Cleanup Kit ensures cleanup of up to 5 μg DNA (70 bp to 4 kb) from enzymatic reactions, delivering high yields of DNA suitable for a range of applications. The kit provides spin columns for cleanup of enzymatic reactions. Using a microcentrifuge or vacuum manifold, high concentration of DNA fragment (70 bp – 4 kb) is quickly achieved. (DNA fragments larger than 4 kb should be purified using the QIAquick System.)

Examples of enzymes that are completely removed by the MinElute Reaction Cleanup Kit
ProteinMolecular weight per enzyme subunit (kDa)
DNA Polymerase I 109
Klenow fragment 62
Calf intestinal alkaline phosphatase 69
T4 DNA ligase 55
T4 Polynucleotide kinase 35
Terminal transferase 32
DNase I 31
Restriction enzymes Varies
原理

MinElute Kits contain a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. The purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples. Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. Specialized binding buffers are optimized for specific applications and promote selective adsorption of DNA molecules within particular size ranges.

Gel loading dye

To enable faster and more convenient sample processing and analysis, gel loading dye is provided. GelPilot Loading Dye contains three tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments migrating too far (see figure "GelPilot Loading Dye").

操作流程

The MinElute system uses a simple bind-wash-elute procedure (see flowchart "MinElute procedure"). Binding buffer is added directly to the enzymatic reaction, and the mixture is applied to the MinElute spin column. The binding buffer contains a pH indicator, allowing easy determination of the optimal pH for DNA binding (see figure "pH Indicator Dye"). Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Handling

MinElute spin columns are designed to provide two convenient handling options (see flowchart "MinElute procedure"). The spin columns fit into a conventional table-top microcentrifuge or onto any vacuum manifold with luer connectors, such as QIAvac 24 Plus or QIAvac 6S with QIAvac Luer Adapters. The MinElute Reaction Cleanup Kit, in addition to other QIAGEN spin-column-based kits, can be fully automated on the QIAcube, enabling increased productivity and standardization of results (see figures "Spin column handling options A, B, C, D, and E").

应用

DNA fragments purified with the MinElute System are ready for direct use in all applications, including:

Sequencing
Microarray analysis
Ligation and transformation
Restriction digestion
Labeling
特点
参数
结合能力 5 µg
洗脱体积 10 µl
规格 Tube
片段大小 70 bp – 4 kb
处理 Manual
回收:寡核苷酸dsDNA Recovery: oligonucleotides, dsDNA
去除<10mers、17–40mers的染料终止子蛋白 Removal <40mers
样本类型:应用 DNA, oligonucleotides: Enzymatic reactions
技术 Silica technology

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安全数据表
1
Download Safety Data Sheets for QIAGEN product components.
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图片
MinElute Procedure
MinElute实验流程。
简单的结合-洗涤-洗脱流程十分便利。
GelPilot Loading Buffer
GelPilot Loading Dye。
GelPilot Loading Dye含有3种示踪染料,有助于优化DNA分辨率。
MinElute Membrane Assembly
MinElute过滤膜的组成。
MinElute离心柱的横断面显示了独特的膜组成。
ILLU_0104_MinElute
pH指示剂染料。
利用溶解液和结合缓冲液中的pH指示剂染料可轻松观测DNA吸附的最佳pH值 (pH ≤7.5)。如果琼脂糖凝胶电泳缓冲液使用频繁或者配制不当,则会出现错误的结合混合物pH值。此时,可加入10 µl 3 M醋酸钠(pH 5.0)轻松调整pH值。
离心柱处理选择 — D。
QIAvac 24 plus。
离心柱处理选择 — E。
QIAcube全自动核酸纯化仪。
QIAprep Spin Column handling options
离心柱处理选择 — C。
带有鲁尔接头的底座。
QIAprep Spin Column handling options
离心柱处理选择 — B。
QIAvac 24。
QIAprep Spin Column handling options
离心柱处理选择 — A。
微型离心机。