For purification of up to 12 µg genomic, mitochondrial, or viral DNA from blood and related body fluids
  • Rapid purification of high-quality, ready-to-use DNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Complete removal of contaminants and inhibitors for reliable results

The QIAamp DNA Blood Mini Kit provides silica-membrane-based DNA purification. The QIAamp DNA Blood Mini Kit is designed for processing up to 200 μl fresh or frozen human whole blood. QIAamp Mini spin columns can be easily processed in a centrifuge or on vacuum manifolds. Purification of DNA using the QIAamp DNA Blood Mini Kit can be fully automated on the QIAcube.

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Produto Num Cat. Preço de lista:
QIAamp DNA Blood Mini Kit (50)
For 50 DNA minipreps: 50 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml)
51104
Cotação
QIAamp DNA Blood Mini Kit (250)
For 250 DNA minipreps: 250 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml)
51106
Cotação

QIAamp DNA Blood Mini Kit are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


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QIAamp spin column procedure.|Apoptotic banding in stored blood.|Long-range PCR.|Paternity testing by RFLP analysis.|The QIAcube.|
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp spin column. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer. The entire process requires only 20 minutes of handling time (lysis times differ according to the sample source).|Genomic DNA from 8 blood samples stored at 4°C for 1 week. DNA was purified using the QIAamp DNA Blood Mini Kit. When blood is stored at 4°C, the DNA is rapidly degraded due to apoptosis; the resulting apoptotic banding pattern can clearly be seen in these samples. M1: lambda-HindIII; M2:100 bp ladder.|Amplification of a 10 kb fragment of the human ALDH1 gene from genomic DNA isolated from blood. DNA was purified using conventional methods (Phenol) or the QIAamp DNA Blood Maxi Kit (QIAamp). PCR products were sequenced directly. M: 1 kb ladder.|Three paternity cases tested with DNA purified from blood samples with the QIAamp DNA Blood Mini Kit. 1: mother; 2: child; 3: alleged father; 4: child + alleged father. M: markers. Each lane had 3 µg DNA loaded. Outcome: A: alleged father excluded; B and C: alleged father confirmed. (Data kindly provided by J. James, Gene Proof Technologies, Nashville, TN, USA.)||
Desempenho

QIAamp DNA Blood Kits yield DNA sized from 200 bp to 50 kb, depending on the age and storage of samples (see figure "Apoptotic banding in stored blood"). The purified DNA is suitable for long-range PCR amplification (see figure "Long-range PCR") and restriction fragment length polymorphism (RFLP) analysis used, for example, for paternity testing (see figure "Paternity testing by RFLP analysis").

Fundamento

No phenol–chloroform extraction is required. DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acid to be eluted in either water or a buffer provided with the kit. QIAamp DNA Blood technology yields genomic, mitochondrial, or viral DNA from blood and related body fluids ready to use in PCR and blotting procedures.

Procedimento

The QIAamp DNA Blood Mini Kit simplifies isolation of DNA from blood and related body fluids with fast spin-column or vacuum procedures (see flowchart "procedure"). Fresh and frozen whole blood with common anticoagulants, such as citrate, EDTA, and heparin, may be processed. The QIAamp DNA Blood Mini Kit processes sample sizes of up to 200 µl, with a preparation time of 20–40 minutes. The typical yield from 200 µl healthy whole blood is 4–12 µg, with an elution volume of 50–200 µl. The QIAamp DNA Blood Mini Kit procedure is automatable (see figure "QIAcube").

QIAamp sample preparation technology is fully licensed.

Vacuum processing

With the QIAamp DNA Blood Mini Kit, blood can be processed by vacuum instead of centrifugation, for greater speed and convenience in DNA purification. QIAamp mini spin columns are accommodated on the QIAvac 24 manifold using VacValves and VacConnectors. VacValves should be used if sample flow rates differ significantly, to ensure consistent vacuum. Disposable VacConnectors are used to avoid any cross-contamination. Use of VacConnectors also allows these QIAamp spin procedures to be performed on QIAvac 6S with QIAvac Luer Adapters.

Aplicações

The QIAamp DNA Blood Mini Kit provides proven QIAamp technology for purification of DNA from a variety of materials using Mini spin columns with 2.0 ml collection tubes. Sample sources include:

Fresh and frozen whole blood or buffy coat
Plasma or serum
Bone marrow
Lymphocytes
Platelets
Body fluids
Característica
Especificações
Applications PCR, long-range PCR, Southern blotting
Elution volume 50–200 µl
Format Spin column
Main sample type Whole blood, body fluids
Processing Manual (centrifugation or vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA, mitochondrial DNA, viral DNA
Sample amount 1–200 µl
Technology Silica technology
Time per run or per prep 20–40 minutes
Yield 4–12 µg

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User-Developed Protocols
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Buffer AS (a special stabilizing reagent available from QIAGEN on request) allows storage of blood samples for up to 12 weeks at temperatures up to 37°C before preparation of DNA with QIAamp® Kits, without any significant reduction in DNA yield or quality. Storage at lower temperatures will extend the potential storage time.
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