For sensitive detection of His-tagged proteins

  • Highly specific and sensitive detection of His-tagged proteins
  • Flexible detection using any steptavidin or avidin conjugate

In detection procedures, the Penta·His Conjugate binds to a protein's His tag and the complex is localized using avidin derivatives, such as streptavidin, which can be conjugated to fluorescent dyes (e.g., streptavidin-R-PE) or to other proteins (e.g., HRP, AP) used in detection procedures.

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Penta·His Biotin Conjugate
125 μl Penta·His Biotin Conjugate, 200 μg/ml
34440
Cotação
The Penta·His Biotin Conjugate is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Desempenho
Performance of Penta·His Biotin Conjugate
PropertyValue
Detection: Five consecutive histidine residues
Antibody isotype: Mouse IgG1
Cross-reactivity: Negligible with crude E. coli, yeast, mammalian, or insect cell lysates
Form: Stabilized stock solution
Working solution: Dependent on application
Fundamento

The Penta·His Biotin Conjugate can be used in conjunction with any streptavidin or avidin conjugate, delivering high flexibility in assay development.

Procedimento
The QIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.
Aplicações

Penta·His Biotin Conjugates allow colorimetric, chromogenic, chemiluminescent, or fluorescent detection of His-tagged proteins in western and dot blot, ELISA and other immunoassay procedures, and fluorescence-activated cell sorting (FACS).

Característica
Especificações
Conjugated enzyme Biotin
Detection Secondary antibody required
Epitope detected HHHHH
Tag His tag

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Kit Handbooks
1
For Anti·His Antibodies, Anti·His HRP Conjugates, Penta·His™ Alexa Fluor® Conjugates, Penta·His Biotin Conjugate, Ni-NTA Conjugates, Tag·100™ Antibody, Streptavidin–R-PE, 6xHis Protein Ladder, Ni-NTA HisSorb™ Strips and Plates 
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