For sensitive, direct immunofluorescent detection of His-tagged proteins, with an emission peak at 532 nm

  • Highly specific and sensitive detection of His-tagged proteins
  • Direct detection without the need for a secondary antibody
  • Broad instrument compatibility and high photostability

Penta·His Alexa Fluor 532 Conjugate is for sensitive, direct immunofluorescent detection of recombinant proteins carrying His tags (consisting of at least five consecutive histidine residues) at 532 nm. Penta·His Alexa Fluor Conjugates are used for applications including immunocytochemistry, protein localization and targeting studies, and fluorescence-activated cell sorting (FACS).

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Penta·His Alexa Fluor 532 Conjugate
125 μl Penta·His Alexa Fluor 532 Conjugate, 200 μg/ml
The Penta·His Alexa Fluor 532 Conjugate is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Performance of Penta·His Alexa Fluor Conjugates
Property Value


Five consecutive histidine residues
Antibody isotype: Mouse IgG1
Cross-reactivity: Negligible with crude E. coli, yeast, mammalian, or insect cell lysates
Form: Stabilized stock solution
Working solution: Dependent on application

Penta·His Antibodies are available as Alexa Fluor 488, 532, 555, and 647 conjugates, giving a range of highly specific reagents whose emission wavelengths cover a wide portion of the visible spectrum.

The QIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.

The Penta·His Alexa Fluor 532 Conjugate can be used in immunofluorescence procedures such as flow cytometry, confocal laser microscopy, immunocytochemistry, immunohistochemistry, protein localization, and targeting studies. This conjugate is highly suited for detection of 6xHis-tagged proteins using the LabMAP bead-based assay system.

Detection Direct
Epitope detected HHHHH
Tag His tag

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Kit Handbooks
For Anti·His Antibodies, Anti·His HRP Conjugates, Penta·His™ Alexa Fluor® Conjugates, Penta·His Biotin Conjugate, Ni-NTA Conjugates, Tag·100™ Antibody, Streptavidin–R-PE, 6xHis Protein Ladder, Ni-NTA HisSorb™ Strips and Plates 
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