AllPrep DNA/RNA/Protein Mini Kit

For simultaneous purification of DNA, RNA, and protein from cells and tissues
  • Maximal recovery of each analyte from precious samples
  • Time savings through short, streamlined workflow
  • Comparable data as analytes come from same sample
  • No need for organic solvents such as phenol and acetone

The AllPrep DNA/RNA/Protein Mini Kit allows simultaneous purification of DNA, RNA, and protein from the same cell or tissue sample. The kit can be used in combination with Allprotect Tissue Reagent, which delivers immediate stabilization of DNA, RNA, and protein in tissue samples for long-term storage without freezing. Tissues can be efficiently disrupted using a TissueRuptor or TissueLyser system.

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製品名 Cat. no. List price:
AllPrep DNA/RNA/Protein Mini Kit (50)
For 50 preps: AllPrep DNA Spin Columns, RNeasy Spin Columns, Collection Tubes, RNase-Free Reagents and Buffers
80004
¥80,000

AllPrep DNA/RNA/Protein Mini Kit  は分子生物学実験用です。疾病の診断、治療または予防の目的には使用することはできません。


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信頼できるリアルタイムPCR/RT-PCR解析|AllPrep DNA/RNA/Protein操作手順|信頼できるウェスタンブロットおよびSDS-PAGE|
ラット組織をAllprotect Reagent 中で安定化し、AllPrep DNA/RNA/Protein Mini Kit を用いてDNAとRNAを精製した。リアルタイムPCRおよびRT-PCR解析におけるCT 値は、液体窒素で安定化した組織で得られたCT 値と同等であった。||ラット組織をAllprotect Reagent 中で安定化し、AllPrep DNA/RNA/Protein Mini Kit を用いてタンパク質を精製した。サンプルをduplicate でSDS-PAGE 電気泳動した後、 [A] ERK2 のウェスタンブロットと [B] Coomassie 染色を行なった。|
パフォーマンス
Genomic DNA and total RNA purified using the AllPrep DNA/RNA/Protein Mini Kit deliver optimal results in real-time PCR and real-time RT-PCR analyses (see figure "Reliable real-time PCR and RT-PCR analyses"). Total protein purified using the kit is suitable for SDS-PAGE and western blotting (see figure "Reliable western blotting and SDS-PAGE").
原理

The AllPrep DNA/RNA/Protein Mini Kit standardizes sample preparation for systems biology. DNA, RNA, and protein are all prepared from the same source, eliminating the variation inherent in preparing these analytes from different samples.There is no need to split the sample into 3 prior to purification, allowing maximum recovery of DNA, RNA, and protein. In addition, no organic solvents such as phenol and acetone are required.

操作手順

Cells or tissues are first lysed and homogenized in Buffer RLT, which immediately inactivates DNases and RNases as well as proteases to ensure isolation of intact DNA, RNA, and proteins. The lysate is then passed through an AllPrep DNA spin column. This column, in combination with the high-salt buffer, allows selective and efficient binding of genomic DNA. The column is washed and pure, ready-to-use DNA is then eluted.

Ethanol is added to the flow-through from the AllPrep DNA spin column to provide appropriate binding conditions for RNA, and the sample is then applied to an RNeasy spin column, where total RNA binds to the membrane and contaminants are efficiently washed away. High-quality RNA is then eluted in RNase-free water.

Buffer APP, a novel aqueous protein precipitation solution, is added to the flow-through of the RNeasy spin column, and the precipitated proteins are pelleted by centrifugation. Intact total proteins are redissolved in an appropriate buffer and then ready to use in downstream applications. The kit includes Buffer ALO, which is compatible with SDS-PAGE, for dissolving the protein pellet. (See flowchart "AllPrep DNA/RNA/Protein procedure".) 

アプリケーション

The AllPrep DNA/RNA/Protein Mini Kit streamlines sample preparation by enabling simultaneous purification of genomic DNA, total RNA, and total protein from the same cell or tissue sample. Total protein purified using the kit is suitable for SDS-PAGE and western blotting. Genomic DNA and total RNA purified using the AllPrep DNA/RNA/Protein Mini Kit deliver optimal results in all downstream applications including:

Real-time PCR analysis
Real-time RT-PCR analysis
Feature
Specifications
Applications PCR, RT-PCR,real-time PCR, real-time RT-PCR, Southern-,Northern-,Western Blotting, Microarrays, Genotyping,1D and 2D gel electrophoresis
Elution volume RNA: 30 - 50µl, DNA: 100 - 200µl
Format Spin column
Main sample type Easy-to-lyse tissue or cells
Processing Manual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Total RNA, genomic DNA and western-grade proteins
Sample amount up to 30 mg tissue or 1 x 10e7 cells
Technology Silica technology, Protein precipitation
Time per run or per prep 60 min
Yield varies, depends on sample type

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キットハンドブック
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For simultaneous purification of genomic DNA, total RNA, and total protein from the same cell or tissue sample
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同一の細胞あるいは組織サンプルからゲノムDNA、トータルRNA、トータルタンパク質を同時に分離精製
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For simultaneous purification of genomic DNA, total RNA and total protein from the same cell or tissue sample
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BC_0047_Allprep
信頼できるリアルタイムPCR/RT-PCR解析
ラット組織をAllprotect Reagent 中で安定化し、AllPrep DNA/RNA/Protein Mini Kit を用いてDNAとRNAを精製した。リアルタイムPCRおよびRT-PCR解析におけるCT 値は、液体窒素で安定化した組織で得られたCT 値と同等であった。
AllPrep DNA/RNA/Protein procedure
AllPrep DNA/RNA/Protein操作手順
Reliable western blotting and SDS-PAGE
信頼できるウェスタンブロットおよびSDS-PAGE
ラット組織をAllprotect Reagent 中で安定化し、AllPrep DNA/RNA/Protein Mini Kit を用いてタンパク質を精製した。サンプルをduplicate でSDS-PAGE 電気泳動した後、 [A] ERK2 のウェスタンブロットと [B] Coomassie 染色を行なった。