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RNAprotect Tissue Tubes (50 x 1.5 ml)
For stabilization of RNA in 50 x 150 mg tissue samples: 50 screw-top tubes containing 1.5 ml RNAprotect Tissue Reagent each
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76154
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RNAprotect Tissue Tubes (20 x 5 ml)
For stabilization of RNA in 20 x 500 mg tissue samples: 20 screw-top tubes containing 5 ml RNAprotect Tissue Reagent each
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76163
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QIAGEN’s former RNA Stabilization Reagent, was manufactured and offered under license from Ambion, Inc., the owner of the RNAlater® trade mark. Following the expiration of that license and corresponding patents, QIAGEN’s new product name for the identical formulation is RNAprotect TissueTubes.
Performance
RNAlater Stabilization Reagent可在收集和储存样本时稳定和保护RNA表达谱(参见"Reagent prevents degradation of mRNA in tissueslater RNA")。RNAlater技术对RNA的立即保护可确保下游分析真实地反映整个组织样本的表达谱(参见"TissueProtect Tubes prevent RNA changes in tissueslater RNA")。样本可长期保存,无RNA降解风险,甚至经多次冻融后也无影响。在从多种条件下保存的RNAlater稳定样本中纯化得到的RNA,适用于northern印迹和RT-PCR等下游应用(参见"Stable RNA in tissues: different temperatures and freeze–thaw cycles")。
Principle
一旦生物样本被收集后,其RNA会变得极不稳定。创新的液状RNAlater RNA Stabilization Reagent可快速渗入组织,立即稳定RNA,保护RNA表达模式。还能避免样本处理导致的基因诱导或下调,可进行准确的基因表达分析。
Procedure
组织样本收集后,迅速浸入适当体积的RNAlater RNA Stabilization Reagent中,即可保护RNA,保存样本。用可重复密封的管子,RNAlater TissueProtect Tubes可预先测定所需RNAlater RNA Stabilization Reagent的体积,方便操作和样本保存。该试剂可使RNA在37°C下保存1天,18–25°C下保存7天,2–8°C下保存4周,并且可以在无干冰或液氮条件下处理、储存和运输样本。另外,样本也可以在–20°C或–80°C的条件下存档。
Applications
使用RNAlater RNA Stabilization Reagent稳定的动物和人类组织可用QIAGEN仪器进行样本破碎,并与QIAGEN试剂盒配合使用,进行:
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总RNA纯化
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miRNA纯化
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mRNA纯化