QIAxcel Advanced System

For effortless DNA and RNA analysis
  • Rapid analysis of up to 96 samples without manual intervention
  • Safety and convenience with ready-to-use gel cartridges
  • Accurate analysis of low concentration nucleic acids with 3–5 bp resolution
  • User-friendly analysis software that supports electronic signatures and records
  • Objective RNA quality measurement with new RNA Integrity Score (RIS)
 
The revolutionary QIAxcel Advanced system replaces traditional, labor-intensive gel analysis of DNA and RNA — streamlining workflows and reducing time to result. The QIAxcel Advanced system fully automates sensitive, high-resolution capillary electrophoresis of up to 96 samples per run. DNA fragment analysis of 12 samples can be performed in as little as 3 minutes. Ready-to-run gel cartridges allow 96 samples to be analyzed with a minimum of hands-on interaction, reducing manual handling errors and eliminating the need for tedious gel preparation. User-friendly QIAxcel ScreenGel software ensures convenient analysis and documentation of data. The QIAGEN RNA Integrity Score (RIS) provides an objective quality measurement of the analyzed samples and allows easy interpretation of sample integrity.

Explore the virtual demo and watch a video to learn more about the QIAxcel Advanced system.

The QIAxcel Advanced system operates in conjunction with the QIAxcel DNA Kits and QIAxcel RNA QC Kit v2.0.
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产品 货号 目录价:
QIAxcel Advanced System
Instrument and service agreement package: workstation for automated capillary electrophoresis, notebook computer and QIAxcel software; includes installation, application training and one-year warranty on labor and parts. Instrument repaired in Regional Repair Center with cost of labor, shipping and parts covered.
9002123
询价
QIAxcel Advanced, Priority
Instrument and service agreement package: workstation for automated capillary electrophoresis, notebook computer and QIAxcel software; includes installation, application training, Full Agreement for two years with two preventive maintenance visits. Instrument repaired in Regional Repair Center with cost of labor, shipping and parts covered.
9002124
询价
QIAxcel Advanced, PrioPLUS
Instrument and service agreement package: workstation for automated capillary electrophoresis, notebook computer and QIAxcel software; includes installation, application training, Full Agreement for three years with three preventive maintenance visits. Instrument repaired in Regional Repair Center with cost of labor, shipping and parts covered.
9002125
询价
QIAxcel ScreenGel Software
Separate license for use of QIAxcel ScreenGel Software on an additional computer
9021163
询价
QIAxcel ScreenGel Software (10)
Ten licenses for use of QIAxcel ScreenGel Software on additional computers
9021165
询价
QIAxcel Advanced System PRV-2
Instrument and service agreement package: workstation for automated capillary electrophoresis, notebook computer and QIAxcel software; includes installation, application training and two inspection service visits. One-year warranty on labor and parts are also included. Instrument is serviced at our Regional Repair Center.
9002874
询价
QIAxcel Advanced, PrioPLUS, no PM
Instrument and service agreement package: workstation for automated capillary electrophoresis, notebook computer and QIAxcel software; includes installation, application training and Full Agreement for three years. Instrument repaired in Regional Repair Center with cost of labor, shipping and parts covered. Does not include preventive maintenance.
9002200
询价
QIAxcel Advanced Instrument
Capillary electrophoresis device: includes computer, QIAxcel ScreenGel Software, and 1-year warranty on parts and labor
9001941
询价

The QIAxcel Advanced instrument is intended to be used only in combination with QIAxcel Kits for the applications described in the respective QIAxcel Kit handbooks.
通过超声检测DNA的降解程度。|操作时间短。|Streamlined workflow.|使用QIAxcel Advanced System进行高效的RNA分析。|RIS能反映RNA的完整性。|对细菌进行准确的高通量基因分型。|分析纯化的RNA。|核酸分离流程。|即用型凝胶卡夹。|简单、标准化的数据采集和分析。|即用型软件。|
[A] 琼脂糖凝胶照片显示,基因组DNA存在不同程度的降解。泳道1:gDNA没有降解的样本;泳道2和3:分别通过7和14脉冲检测到gDNA有部分降解的样本;泳道4和5:通过70和105脉冲检测到gDNA完全降解的样本。[B] QIAxcel凝胶照片显示样本的gDNA存在三种状态的降解。[A]中的1、3和5对应泳道1、3和5。[C]电泳凝胶照片表明gDNA具有高品质(在主峰前无DNA降解,长拖尾),泳道3:DNA部分降解(有部分降解产物的信号,没有拖尾),泳道5:gDNA严重降解(许多降解产物信号,没有拖尾)。|在QIAxcel Advanced System上使用QIAxcel DNA High Resolution Kit进行核酸分离所需的时间少于传统的高分辨率琼脂糖分离所需时间的10%。||从Schizosaccharomyces pombe中纯化总RNA。结果如图所示[A]凝胶图像[B]叠加电泳图。对酵母中总RNA的未标记cRNA进行可靠的质量控制。结果如图所示[C]凝胶图像[D]叠加电泳图。|在降解条件下,使用RNeasy Plus Mini Kit从大鼠肝脏样本中纯化总。在QIAxcel Advanced和Agilent 2100 Bioanalyzer上分析样本的降解成都。[A]为QIAxcel Advanced的凝胶图片,显示分析结果,[B]为Agilent Bioanalyzer的分析结果。QIAxcel Advanced的电泳分析图为[C]。RIS (Qiaxcel Advanced)和RIN(Agilent Bioanalyzer)列在表格中。|样本在[A]QIAxcel Advanced System上以初始浓度进行分析,或在[B]琼脂糖凝胶上以五倍浓度进行电泳分析。样本3[C]和5[D]以初始浓度(O)和五倍浓度(C)在琼脂糖上进行电泳分析,并且以初始浓度在QIAxcel Advanced(Q)上进行分析。数据由加拿大阿尔伯塔省,加拿大公共卫生署食源性人畜共患病重点实验室Mutschall及其同事提供。|纯化总RNA的电泳图。数据由荷兰乌特勒支大学医学中心微阵列实验室的D. van Leenen提供。|通过对充满凝胶的毛细管施加电流,使核酸分子按照不同大小分离开来,在其向阳极移动的过程中被分别检测。信号数据通过光电倍增管,被QIAxcel ScreenGel Software转换为电泳图和凝胶图。|QIAxcel凝胶卡夹将接触有害化学物的风险降至最低。|QIAxcel ScreenGel软件为您简化数据分析工作。|QIAxcel ScreenGel软件为您简化实验设置和数据分析。|
性能
Streamlined workflow
The QIAxcel Advanced system not only speeds up DNA and RNA analysis by eliminating slab-gel analysis, but also contributes to streamlining the entire sample purification and analysis workflow. Used in combination with proven QIAGEN end-point PCR kits, the QIAxcel Advanced provides an all-in-one, pretested solution for reliable analysis of PCR fragments, ensuring reproducibility and significant time and cost savings.

One instrument, many applications
The QIAxcel Advanced system is a versatile solution for electrophoresis and offers a broad range of applications. Preprogrammed methods, in combination with the corresponding gel cartridges, allow separation and analysis of a variety of nucleic acids — including single or multiple PCR fragments, DNA digested with restriction endonucleases, total RNA, and cRNA. Unlike other commercially available devices that provide processing of only up to 12 samples at time, the QIAxcel Advanced system allows processing of all 96 samples without manual intervention.

DNA analysis
In genotyping applications, the QIAxcel Advanced system enables greater sizing accuracy and more sensitive detection than conventional agarose gel electrophoresis (see figure Accurate, high-throughput genotyping of bacteria). QIAxcel Advanced also provides a straightforward and effective means for checking the quality of genomic DNA (gDNA) samples prior to molecular screening and downstream assays (see figure Assessment of DNA degraded by sonication).

RNA analysis — objective quality values with the RNA Integrity Score
The new QIAGEN RNA Integrity Score (RIS) provides an objective quality measurement for eukaryotic RNA samples and allows easy interpretation of sample integrity. The RIS is a value from 1 to 10 where a value of 10 indicates completely intact RNA (see figure RIS indicates RNA integrity). The highly reproducible RIS eliminates the need for human interpretation and enables the implementation of rigorous quality control at appropriate breakpoints across workflows. This allows researchers to eliminate samples that do not meet the quality criteria required for downstream processes, reducing the risk of needing to repeat experiments and saving time and costs.

Such careful monitoring of the initial RNA sample, as well as products generated throughout the entire procedure, is crucial in microarray analysis, since RNA degradation strongly influences the predictive power obtained from the microarray data. The QIAxcel Advanced System is highly suitable for analyzing the integrity of total RNA and size distribution of fragmented or intact cRNA (see figure Streamlined RNA analysis using the QIAxcel system), and the throughput capacity makes it very convenient for laboratories employing 96-well RNA purification technologies that need a fast solution for quality control.

原理
The QIAxcel Advanced instrument includes an array of light-emitting diodes and micro-optical collectors that latch to capillaries within QIAxcel gel cartridges. Fragments that are migrating through a gel matrix within the capillary pass excitation and detection spots and the signal is transmitted through a photomultiplier tube to the QIAxcel ScreenGel software for data interpretation (see figure Sample separation process).

The high detection sensitivity provided by the QIAxcel Advanced instrument enables robust results even with low concentrations of nucleic acid. With a resolution of 3–5 bp for fragments smaller than 0.5 kb, the QIAxcel Advanced system ensures greater accuracy than slab-gel methods, as well as greater confidence in data interpretation. Sample consumption is less than 0.1 μl per analysis, saving precious samples for further downstream analysis. Hands-free sample loading and self-contained components minimize exposure to hazardous chemicals, such as ethidium bromide (see figure Ready-to-run gel cartridges).

A range of QIAxcel Kits is available for a variety of applications, meeting different resolution and speed requirements (see table below).

QIAxcel Kits
QIAxcel Kit Analyte Size range 100 bp – 500 bp* 500 bp – 1 kb* 1 kb – 5 kb* 5 kb – 10 kb* Run time/
12 samples
QIAxcel DNA High Resolution Kit DNA 15 bp–10 kb 3–5 bp 50 bp 200–500 bp 1–1.5 kb 7–20 min
QIAxcel DNA Screening Kit DNA 15 bp–5 kb 20–50 bp 50–100 bp 500 bp 5 min
QIAxcel DNA Fast Analysis Kit DNA 15 bp–3 kb 50–100 bp 100–250 bp 250 bp – 1 kb 3–5 min
QIAxcel RNA QC Kit v2.0 RNA 15 b–6 kb 10 min
* Best resolution. Run time depends on method used. Best resolution between 1–3 kb.
操作流程
Just a few, simple steps are required to operate the QIAxcel Advanced system for analysis of DNA and RNA samples: load the gel cartridge, fill and load the buffer tray, load the samples in 96-well plates or in PCR tubes or strips, select the process profile to be used — and go! Tedious gel preparation and extensive user training are eliminated, streamlining the workflow and facilitating integration of the system into the daily lab routine. Within minutes of starting a run, the first results appear in real time on the computer screen (see figure Reduced run times).
应用

The QIAxcel Advanced System offers a broad range of optimized applications for DNA and RNA analysis and is highly suited for research in academic as well as pharmaceutical, biotechnology, and biomedical laboratories. Preprogrammed methods, in combination with the corresponding gel cartridges, allow separation and analysis of a variety of nucleic acids — including single or multiple PCR fragments, DNA digested with restriction endonucleases, synthesized oligonucleotides, total RNA, and cRNA.

The QIAxcel Advanced System has been used for applications such as:

Bacterial genotyping
PCR fragment analysis
Pre-sequencing testing
Mutation detection
Detection of food allergens
Quality control of genomic DNA
Total RNA and cRNA QC for RT-PCR and microarray analysis
服务

Have your throughput demands increased? Do you want to expand your application range or further streamline workflows? Do you need to manage more complex samples? Do you need improved connectivity?

If the answer to any of these questions is yes, then QIAGEN’s trade-in/trade-up program is just right for you!

QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply exchange an old instrument for a new one. Improve your efficiency by trading in a competitor instrument or trading up with an older QIAGEN instrument.

Learn more about trade-in/trade-up opportunities.

软件
QIAxcel ScreenGel software provides a new level of convenience
QIAxcel ScreenGel software, specifically developed for use with the QIAxcel Advanced system, is a powerful and user-friendly tool for data collection and analysis. Interactive tools simplify analysis, facilitate rapid data interpretation, and provide flexibility with data and results displayed in both electropherogram and gel image formats. Results can be viewed individually or displayed as overlay views for sample and data comparison. All-in-one analysis for multiple data sets simplifies evaluation. A unique algorithm calculates and generates a tabular display of a variety of peak properties, including number of peaks as well as the size, height, width, and area of each peak. Comprehensive data reports can be easily generated and saved or exported to meet individual documentation needs. Complete process profiles for standardized sample processing — from running samples to data analysis, generating reports and exporting data — minimize the need for additional training for users (see figure Simplified and standardized data collection and analysis).

Easy setup and start
The Process Wizard simplifies experiment startup (see figure Easy-to-use software features). This convenient feature enables guided setup and allows run parameters to be defined and DNA size markers to be selected. Reagent lot number information can be included. Samples can be selected with a single click of the mouse and run checks can also be easily performed.

New RNA Integrity Score
The updated QIAxcel ScreenGel software now features the QIAGEN RNA Integrity Score (RIS), which enables objective quality measurement of analyzed samples and allows easy interpretation of sample integrity. The RIS is a value from 1 to 10 where a value of 10 indicates completely intact RNA (see figure RIS indicates RNA integrity). The highly reproducible RIS eliminates the need for human interpretation and enables the implementation of rigorous quality control at appropriate breakpoints across workflows. This allows researchers to eliminate samples that do not meet the quality criteria required for downstream processes, reducing the risk of needing to repeat experiments and saving time and costs.

Support of 21 CFR Part 11 compliance
QIAxcel ScreenGel software includes specific features that support the technical requirements of 21 CFR Part 11 regulations. Security features such as the following are available:
Password-protected login to prevent unauthorized access and data manipulation
Audit trail documentation for configuration files and system events
Automatic saving and archiving of write-protected raw data
Secure user management

Different user profiles are available (Routine, Basic, Advanced, Admin) and are password protected (user login is required) for increased security. A simplified interface minimizes the need for user training and makes the software especially attractive for inexperienced users.

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应用说明
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The QIAxcel System was successfully used to detect DNA derived from genetically modified organisms (GMOs) at a level suitable for GMO testing according to EU standards.
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For effortless DNA and RNA analysis
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产品介绍与指南
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For analysis and typing of PCR products from different HLA loci using the QIAxcel Advanced and the Helmberg-SCORE software


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For evaluation of samples for next-generation sequencing (NGS) on the QIAGEN GeneReader instrument
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A protocol for evaluating quality of RNA samples using the QIAxcel Advanced Instrument and ScreenGel software version 1.3 or higher
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For evaluation of samples for next-generation sequencing (NGS) on the QIAGEN GeneReader platform using ScreenGel Software version 1.5 or higher
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Addressing critical factors and new solutions
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A protocol for evaluation of samples for next-generation sequencing on the QIAGEN GeneReader platform using the QIAxcel Advanced Instrument and ScreenGel software version 1.5 of higher
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试剂盒操作手册
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For optimal DNA size determination
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For optimal RNA fragment size determination
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For automated analysis of DNA fragments using the QIAxcel and QIAxcel Advanced instruments
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For calibration of signal intensity
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For optimal use of the QIAxcel Gel Cartridges
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For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments
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用户使用手册
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配套QIAxcel Advanced instruments和QIAxcel ScreenGel Software version 1.6
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仪器技术参数
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For easy upgrade of QIAxcel ScreenGel Software Version 1.6.


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QIAxcel® Advanced System and 21 CFR Part 11 Regulations
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操作软件
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This software may only be downloaded by registered users with a valid QIAxcel ScreenGel Software license. If you do not have a valid software license, contact your QIAGEN sales representative.
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This release includes the first version of QIAxcel ScreenGel NGS Profiles. These profiles are intended to be used to run and analyze reactions of GeneRead QIAact AIT and BRCA 1/2 panels using the QIAxcel instrument and QIAxcel ScreenGel 1.5 Software. These profiles are composed of an Process Profile, Analysis Profile, Distribution Profile, and Report/Export Profile.
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其他资源
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Process Profile and Method file for use with QIAxcel ScreenGel Software
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Alignment marker file QX15/600 bp - English (XAM)

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Method file for use with QIAxcel ScreenGel Software
 
 


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Method file for use with QIAxcel ScreenGel Software
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Method file for use with BioCalculator Software
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Method file for use with BioCalculator Software
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Method file for use with BioCalculator Software
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Method file for use with QIAxcel ScreenGel Software
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常见问题
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图片
Assessment of DNA degraded by sonication.
通过超声检测DNA的降解程度。
[A] 琼脂糖凝胶照片显示,基因组DNA存在不同程度的降解。泳道1:gDNA没有降解的样本;泳道2和3:分别通过7和14脉冲检测到gDNA有部分降解的样本;泳道4和5:通过70和105脉冲检测到gDNA完全降解的样本。[B] QIAxcel凝胶照片显示样本的gDNA存在三种状态的降解。[A]中的1、3和5对应泳道1、3和5。[C]电泳凝胶照片表明gDNA具有高品质(在主峰前无DNA降解,长拖尾),泳道3:DNA部分降解(有部分降解产物的信号,没有拖尾),泳道5:gDNA严重降解(许多降解产物信号,没有拖尾)。
Reduced run times
操作时间短。
在QIAxcel Advanced System上使用QIAxcel DNA High Resolution Kit进行核酸分离所需的时间少于传统的高分辨率琼脂糖分离所需时间的10%。
Streamlined workflow
Streamlined workflow.
Streamlined RNA analysis using the QIAxcel Advanced System.
使用QIAxcel Advanced System进行高效的RNA分析。
Schizosaccharomyces pombe中纯化总RNA。结果如图所示[A]凝胶图像[B]叠加电泳图。对酵母中总RNA的未标记cRNA进行可靠的质量控制。结果如图所示[C]凝胶图像[D]叠加电泳图。
RIS indicates RNA integrity.
RIS能反映RNA的完整性。
在降解条件下,使用RNeasy Plus Mini Kit从大鼠肝脏样本中纯化总。在QIAxcel Advanced和Agilent 2100 Bioanalyzer上分析样本的降解成都。[A]为QIAxcel Advanced的凝胶图片,显示分析结果,[B]为Agilent Bioanalyzer的分析结果。QIAxcel Advanced的电泳分析图为[C]。RIS (Qiaxcel Advanced)和RIN(Agilent Bioanalyzer)列在表格中。
Accurate, high-throughput genotyping of bacteria
对细菌进行准确的高通量基因分型。
样本在[A]QIAxcel Advanced System上以初始浓度进行分析,或在[B]琼脂糖凝胶上以五倍浓度进行电泳分析。样本3[C]和5[D]以初始浓度(O)和五倍浓度(C)在琼脂糖上进行电泳分析,并且以初始浓度在QIAxcel Advanced(Q)上进行分析。数据由加拿大阿尔伯塔省,加拿大公共卫生署食源性人畜共患病重点实验室Mutschall及其同事提供。
Analysis of purified RNA
分析纯化的RNA。
纯化总RNA的电泳图。数据由荷兰乌特勒支大学医学中心微阵列实验室的D. van Leenen提供。
Sample separation process
核酸分离流程。
通过对充满凝胶的毛细管施加电流,使核酸分子按照不同大小分离开来,在其向阳极移动的过程中被分别检测。信号数据通过光电倍增管,被QIAxcel ScreenGel Software转换为电泳图和凝胶图。
即用型凝胶卡夹。
QIAxcel凝胶卡夹将接触有害化学物的风险降至最低。
Process profiles simplify and standardize data collection and analysis
简单、标准化的数据采集和分析。
QIAxcel ScreenGel软件为您简化数据分析工作。
Easy to use software features
即用型软件。
QIAxcel ScreenGel软件为您简化实验设置和数据分析。