When running RNA samples using the QIAxcel System or QIAxcel Advanced, are there any special things to consider?
FAQ ID -1842

When running RNA samples on the QIAxcel System or QIAxcel Advanced, the standard RNA denaturation process has to be followed prior to running the sample.

  • Add an equal volume of QX RNA Denaturation buffer to your RNA sample and/or QX RNA size Marker.
  • Heat the solution at 70°C for 2 minutes on a heating block or in a PCR machine, then place on ice for 1 minute.
  • Bring the total sample volume to 10 μl using QX RNA Dilution Buffer and mix the solution by gently pipetting up and down a few times.
  • Analyze the samples immediately.