Duplicate 47 µm filter membranes were used to filter 500 ml of coastal ocean water. Resulting DNA and RNA yields were quantified using a Qubit Fluorometer. Purity was assessed using a NanoDrop Spectrophotometer. MCE: mixed cellulose ester; PES: polyethersulfone; PC: polycarbonate.
Duplicate mixed cellulose ester (MCE) membranes were used to filter water samples until the flow stopped. Resulting DNA and RNA yields were quantified using a Qubit Fluorometer. Purity was assessed using a NanoDrop Spectrophotometer.
B. subtilis culture was incubated overnight after being spotted onto 25 mm filter membranes (200 µl). Another culture was stored at 4°C, the same amount, as a control. MCE: mixed cellulose ester; PES: polyethersulfone; PC: polycarbonate.