Using the DNeasy PowerWater Sterivex Kit, ready-to-use DNA is obtained for any downstream application in just 40 minutes. The protocol includes cell lysis and DNA purification. Other protocols using enzymatic in-unit digestion and phenol/chloroform extraction take longer than 20 hours to complete. These protocols include long incubation, enzymatic digestion and purification protocol times. The DNeasy PowerWater Sterivex Kit provides cell lysis through bead-based homogenization, speeding up the process which provides faster results.
Less RNA carryover and higher yields of high molecular weight and dsDNA can be seen in DNA obtained using the DNeasy PowerWater Sterivex Kit compared with other lysis protocols which use phenol/cholorform extraction.
The DNeasy PowerWater Sterivex Kit removes all PCR inhibitors from DNA allowing greater precision in downstream applications. DNA purified through an in-unit digestion protocol followed by phenol/chloroform extraction failed to amplify without a 1:10 dilution.