DNA and RNA was prepared from eight different human stool samples (200 mg each) using a standard protocol (AllPrep DNA/RNA Mini Kit, without IRT) and with the AllPrep DNA/RNA PowerFecal Kit (with IRT). DNA and RNA purified using the AllPrep PowerFecal DNA/RNA Kit shows no inhibition in a PCR assay.
DNA from human, mouse, sheep and horse stool samples (200 mg each) was prepared using the AllPrep DNA/RNA Power Fecal Kit and sheared to 300 bp fragments. Paired-end NGS libraries were prepared, quantified and sequenced. BWA-MEM Version 0.7.10 was used to map DNA reads against the human reference genome Hg19, resulting in a “human DNA-free” data set. Unmapped reads were subsequently mapped against a collection of bacterial genomes.
